• 10 května, 2021

NCBI Reference Sequence (RefSeq):

NCBI Reference Sequence (RefSeq): upravená neredundantní databáze sekvencí genomů, transkriptů a proteinů.

 The Nationwide Heart for Biotechnology Info (NCBI) Reference Sequence (RefSeq) database (http://www.ncbi.nlm.nih.gov/RefSeq/) gives a non-redundant assortment of sequences representing genomic information, transcripts and proteins. Though the objective is to supply a complete dataset representing the whole sequence data for any given species, the database pragmatically consists of sequence information which can be presently publicly accessible within the archival databases. The database incorporates information from over 2400 organisms and consists of over a million proteins representing vital taxonomic variety spanning prokaryotes, eukaryotes and viruses.

Nucleotide and protein sequences are explicitly linked, and the sequences are linked to different assets together with the NCBI Map Viewer and Gene. Sequences are annotated to incorporate coding areas, conserved domains, variation, references, names, database cross-references, and different options utilizing a mixed strategy of collaboration and different enter from the scientific neighborhood, automated annotation, propagation from GenBank and curation by NCBI employees.



EUR 170.4


30-003-CF 50 mL/pk
EUR 97.2
Description: Media Catalog; Cell Culture Reagents


30-006-CF 50 mL/pk
EUR 117.6
Description: Media Catalog; Cell Culture Reagents

DAPI (hydrochloride)

C3362-10 10 mg
EUR 146.4

DAPI (hydrochloride)

C3362-25 25 mg
EUR 240

DAPI (hydrochloride)

C3362-5 5 mg
EUR 111.6

DAPI Dilactate

17509 25 mg
EUR 158.4

DAPI (dihydrochloride)

HY-D0814 50mg
EUR 439.2

Amphotericin B (Fungizone) Sol, 250 µg/mL

CCM1120-100 100 mL
EUR 132.28

Fluorguard Antifade Mounting Medium

F001 1 ml
EUR 103.2

Latex-in-a-Box Conjugation Kit, Blue 0.3 micron, 7 mL

LIB03-B007 7 mL
EUR 880.73
Description: Latex-in-a-Box Conjugation Kit conjugates blue 0.3 micron latex nanoparticles to antibodies and proteins. The nanoparticles are created using a special technology that needs only one hour to conjugate the particles to the antibody/protein.

Latex-in-a-Box Conjugation Kit, Red 0.3 micron, 7 mL

LIB03-R007 7 mL
EUR 880.73
Description: Latex-in-a-Box Conjugation Kit conjugates red 0.3 micron latex nanoparticles to antibodies and proteins. The nanoparticles are created using a special technology that needs only one hour to conjugate the particles to the antibody/protein.

FluorLast? with DAPI

EUR 183.6

DAPI Staining Kit

abx097206-1Kit 1 Kit
EUR 276

Penicillin-Streptomycin 100X Solution, 10, 000 Units/mL, 10, 000 µg/mL

CCM1133-100 100 mL
EUR 76.86

Starch Indicator 0.3%

ST0305 500ML
EUR 93.48

DAPI (Molecular Biology Grade)

CE117 5 mg
EUR 72

DAPI (Molecular Biology Grade)

CE118 25 mg
EUR 159.6

DAPI (Molecular Biology Grade)

CE119 100 mg
EUR 382.8


30-009-CI 100 mL/pk
EUR 96
Description: Media Catalog; Cell Culture Reagents

FluorLast? with DAPI and DABCO

EUR 183.6

Human Paraoxonase 1 (PON) Standards QQ and RR Phenotypes (2 x 0.3 mL)

0801384 2 x 0.3 mL
EUR 396.96
Description: Please contact Gentaur in order to receive the datasheet of the product.

EverBrite Hardset Mounting Medium with DAPI

23004 10mL
EUR 255.6
Description: Minimum order quantity: 1 unit of 10mL

FluoroQuest™ Mounting Medium with DAPI

20004 50 mL
EUR 158.4

Anti-Fade Mounting Medium (With DAPI)

abx090690-10ml 10 ml
EUR 243.6

Brilliant Cresyl Blue (0.3%, Alcoholic)

BCA3800 1 Gal.
EUR 289.2

Brilliant Cresyl Blue (0.3%, Alcoholic)

BCA500 500 ml
EUR 108

Brilliant Cresyl Blue (0.3%, Alcoholic)

BCA999 1000 ml
EUR 139.2

Hydrogen Peroxide 0.3% w/v

HP035 5L
EUR 120.84

Hydrogen Peroxide 0.3% w/v

HP03F 1L
EUR 43.32

Random Hexamers 100 µg

26-4000-03 100 ug
EUR 150

Random Primers are a mixture of oligonucleotides representing all possible sequence for that size. Random Primers can be used to prime synthesis in oligo-labeling similar to using hexamers (1,2) and cDNA synthesis. Random prime labeling yields high specific activity labeled DNA probe which can be used for all southern, northern and in situ hybridization studies. Random Primers can be also used similar to using hexamers in cDNA synthesis in combination with oligo d(T) to yield more 5' end cDNA sequence. Recently random primers have been used to detect DNA polymorphism. These polymorphisms, simply detected as DNA segments which amplify from one parent but not the other, are inherited in a Mendelian fashion and can be used to construct genetic maps in a variety of species. The authors suggested that these polymorphisms be called RAPD (pronounced RAPID) makers, after Random Amplified Polymorphic DNA (3).

References 1. Feinberg, A.P. & Vogelstein, B. (1983) Anal. Biochem. 132:6-13. 2. Feinberg, A.P. & Vogelstein, B. (1984) Anal. Biochem. 137:266-267. 3. Williams J. G., Kubelik A.R., Livak K.J., Rafalski J.A. & Tingey S.V. (1990) Nucleic Acid Res. 18(22):6531-5.

0.3 ml 8-Strip Tubes & Domed Caps, 250 Strips of 8 Tubes and Caps

GP209 2,000 tubes/caps
EUR 400.8

DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride)

B551 0.2 ml
EUR 129.6

DiagPoly Amine Polystyrene Particles, 0.3 µm

DNM-F002 10 mL
EUR 696

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *10 mM solution in water*

17507 2 mL
EUR 138

In Situ Plate Seals, 10 seals

EUR 160
Description: In Situ Plate Seals, 10 seals

Modular In Situ-1 96 Well Crystallization Plate

EUR 392
Description: Modular In Situ-1 96 Well Crystallization Plate

FluoroQuest™ Anti-fading Mounting Medium with DAPI

20005 20 mL
EUR 211

DiagPoly Plain Polystyrene Particles, 0.2-0.3 µm

DNM-P003 10 mL
EUR 664.8

Anti-PON1 Clone KRJ1 (100 µg)

0801012 100 µg
EUR 340.8
Description: Please contact Gentaur in order to receive the datasheet of the product.

Anti-PON1 Clone KRJ2 (100 µg)

0801014 100 µg
EUR 340.8
Description: Please contact Gentaur in order to receive the datasheet of the product.

bFGF-nAb Purified Protein 25 µg

ABP-NAB-BFGFAB 25 µg Ask for price

bFGF-nAb Plus Biotinylated 25 µg

ABP-NAB-BFGFPB 25 µg Ask for price

GFP-nAb Purified Protein 25 µg

ABP-NAB-GFPAB 25 µg Ask for price

GFP-nAb Plus Biotinylated 25 µg

ABP-NAB-GFPPB 25 µg Ask for price

mNeonGreen-nAb Purified Protein 25 µg

ABP-NAB-MNGAB 25 µg Ask for price

mNeonGreen-nAb Plus Biotinylated 25 µg

ABP-NAB-MNGPB 25 µg Ask for price

RFP-nAb Purified Protein 25 µg

ABP-NAB-RFPAB 25 ug Ask for price

RFP-nAb Plus Biotinylated 25 µg

ABP-NAB-RFPPB 25 µg Ask for price

BrightGreen miRNA qPCR MasterMix-Low ROX

MasterMix-mL 4 x 1.25 ml for 500 reactions
EUR 181.2

Rat Monoclonal Anti-Mouse CD4 Pur. Low Endotoxin (Clone YTS 191.1) (rat IgG2b)

MCD004-ML 100 ug
EUR 489.6

Rat Monoclonal Anti-Mouse CD25 , Pur. Low Endotoxin (Clone PC61.5.3) (rat IgG1)

MCD025-ML 100 ug
EUR 489.6

DAPI, dilactate (4’, 6-diamidino-2-phenylindole, dilactate)

40009 10MG
EUR 175.2
Description: Minimum order quantity: 1 unit of 10MG

Kinesis Glyphosate 1 mL 0.1 mg/mL in Water

CHR3649 1ML
EUR 310.08

DiagPoly Streptavidin Coated Polystyrene Particles, 0.3-0.4 µm

DNM-C005 10 mL
EUR 811.2

Sodium Hypochlorite 0.3% w/v available Chlorine - 1L

SH03WW1 1L
EUR 13.68

1000ug/mL Azithromycin in Acetonitrile

LCS-5882 1ML
EUR 381.9

Anilazine 1000ug/mL in MeOH

EUR 92.34

Anilazine 2000ug/mL in MeOH

EUR 134.52

Azobenzene 1000ug/mL in MeOH

EUR 96.9

Azobenzene 2000ug/mL in MeOH

EUR 118.56

Benzidine 1000ug/mL in MeOH

EUR 139.08

Benzidine 2000ug/mL in MeOH

EUR 193.8

Dimethylaminoazobenzene 1000ug/mL in MeOH

EUR 118.56

Dimethylaminoazobenzene 2000ug/mL in MeOH

EUR 139.08

Aniline 1000ug/mL in MeOH

EUR 79.8

Aniline 2000ug/mL in MeOH

EUR 98.04

1000ug/mL Estriol in MeOH

S-5541 1ML
EUR 112.86

1000ug/mL Progesterone in MeOH

S-5543 1ML
EUR 104.88

1000ug/mL Metronidazole in MeOH

S-5570 1ML
EUR 131.1

1000ug/mL Methacrifos in MeOH

S-5619 1ML
EUR 221.16

1000ug/mL Clofentezine in MeOH

S-5772 1ML
EUR 121.98

1000ug/mL Dinotefuran in MeOH

S-5780 1ML
EUR 229.14

1000ug/mL Fenpropathrin in MeOH

S-5781 1ML
EUR 90.06

1000ug/mL Tylosin in MeOH

S-5878 1ML
EUR 124.26

1000ug/mL Adiponitrile in MeOH

S-5932 1ML
EUR 78.66

1000ug/mL Dichlorfenthion in MeOH

S-1280 1ML
EUR 77.52

1000ug/mL Fensulfothion in MeOH

S-2005 1ML
EUR 82.08

1000ug/mL Imidan in MeOH

S-2245 1ML
EUR 71.82

1000ug/mL Prophos in Acetone

S-3185 1ML
EUR 82.08

1000ug/mL Naphtha in MeOH

S-3861A 1ML
EUR 131.1

1000ug/mL Hexamethylcyclotrisiloxane in Hexane

S-4334 1ML
EUR 71.82

1000ug/mL Carbofuranphenol in MeOH

S-4346 1ML
EUR 150.48

1000ug/mL Triclosan in MeOH

S-4382 1ML
EUR 74.1

1000ug/mL Danofloxacin in MeOH

S-4575 1ML
EUR 103.74

1000ug/mL Sulfadimethoxine in MeOH

S-4589 1ML
EUR 136.8

1000ug/mL Trimethoprim in MeOH

S-4594 1ML
EUR 71.82

1000ug/mL Benfuracarb in MeOH

S-4678 1ML
EUR 212.04

1000ug/mL Ethephon in MeOH

S-4682 1ML
EUR 82.08

1000ug/mL Vamidothion in MeOH

S-4695 1ML
EUR 126.54

1000ug/mL Cholesterol in MeOH

S-4702 1ML
EUR 82.08

1000ug/mL Anthanthrene in MeOH

S-4739 1ML
EUR 238.26

1000ug/mL Histamine in MeOH

S-4765 1ML
EUR 90.06

1000ug/mL Pentachloroaniline in MeOH

S-4783 1ML
EUR 79.8

1000ug/mL Melamine in MeOH

S-4806 1ML
EUR 88.92

1000ug/mL Ditalimfos in MeOH

S-4827 1ML
EUR 456

1000ug/mL Diethofencarb in MeOH

S-4830 1ML
EUR 71.82

1000ug/mL Trifloxystrobin in MeOH

S-4836 1ML
EUR 163.02

1000ug/mL Mecarbam in MeOH

S-4847 1ML
EUR 71.82

1000ug/mL Cyproconazole in MeOH

S-4863 1ML
EUR 98.04

1000ug/mL Epoxiconazole in MeOH

S-4923 1ML
EUR 225.72

1000ug/mL Fenhexamid in MeOH

S-4962 1ML
EUR 136.8

1000ug/mL Indoxacarb in MeOH

S-4963 1ML
EUR 106.02

1000ug/mL Triflumizole in MeOH

S-4964 1ML
EUR 226.86

1000ug/mL Tralomethrin in MeOH

S-4965 1ML
EUR 136.8

1000ug/mL Sulfosulfuron in MeOH

S-4997 1ML
EUR 121.98

1000ug/mL Aclonifen in MeOH

S-5154 1ML
EUR 77.52

Butyldiphenylphosphate 1000ug/mL in IPA

EUR 565.44

Decabromodiphenylethane 50ug/mL in Chlorobenzene

EUR 1140

Dibromoneopentylglycol 50ug/mL in IPA

EUR 307.8

Dibutylphenylphosphate 1000ug/mL in IPA

EUR 1218.66

Pentabromoethylbenzene 50ug/mL in Isooctane

EUR 307.8

Anti-Zika NS1 Clone 1D12 (100 µg)

0801025 100 µg
EUR 340.8
Description: Please contact Gentaur in order to receive the datasheet of the product.

Anti-Zika Envelope Clone 4C7 (100 µg)

0801026 100 µg
EUR 340.8
Description: Please contact Gentaur in order to receive the datasheet of the product.

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*

17510 10 mg
EUR 138

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*

17511 100 mg
EUR 262.8

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*

17513 25 mg
EUR 158.4

Human 4’,6-Diamidino-2-phenylindole dihydrochloride(DAPI)ELISA kit

QY-E05538 96T
EUR 433.2

comb 0.25 mm thick 48 sample

ESEQ1100-C48-0.3 ea
EUR 118.8

comb 0.25 mm thick 96 sample

ESEQ1100-C96-0.3 ea
EUR 118.8

spacers 0.25 mm thick 45 cm pk/2

ESEQ1100-SP-0.3 ea
EUR 67.2

comb 0.25 mm thick 24 sample

ESEQ1200-C24-0.3 ea
EUR 87.6

comb 0.25 mm thick 48 sample

ESEQ1200-C48-0.3 ea
EUR 87.6

spacers 0.25 mm thick 45 cm pk/2

ESEQ1200-SP-0.3 ea
EUR 67.2

DiagPoly Plain Fluorescent Polystyrene Particles, Yellow, 0.1-0.3 µm

DNM-L002 2 mL
EUR 788.4

DiagPoly Plain Fluorescent Polystyrene Particles, Pink, 0.1-0.3 µm

DNM-L012 2 mL
EUR 788.4

DiagPoly Plain Fluorescent Polystyrene Particles, Purple, 0.1-0.3 µm

DNM-L015 2 mL
EUR 788.4

DiagPoly Amine Fluorescent Polystyrene Particles, Yellow, 0.09-0.3 µm

DNM-L056 2 mL
EUR 832.8

DiagPoly Carboxyl Fluorescent Polystyrene Particles, Yellow, 0.09-0.3 µm

DNM-L063 2 mL
EUR 789.6

Papain Solution In PBS, 1mg/ml

CA093-002 20ml Ask for price

Acridine Orange, 10mg/mL in water

40039 10ML
EUR 121.2
Description: Minimum order quantity: 1 unit of 10ML

Hoechst 33258, 10mg/mL in H2O

40044 10ML
EUR 260.4
Description: Minimum order quantity: 1 unit of 10ML

Hoechst 33342, 10mg/mL in H2O

40046 10ML
EUR 278.4
Description: Minimum order quantity: 1 unit of 10ML

Ethidium bromide, 10mg/ml in water

GT9211-10ML 10 ml
EUR 84

1000 ug/mL Cyanophos in Acetonitrile

LCS-1006 1ML
EUR 193.8

1000 ug/mL Abate in Acetonitrile

LCS-102 1ML
EUR 152.76

1000 ug/mL Dicrotophos in Acetonitrile

LCS-1500 1ML
EUR 185.82

1000 ug/mL Dimethoate in Acetonitrile

LCS-1560 1ML
EUR 98.04

1000 ug/mL Famphur in Acetonitrile

LCS-1995 1ML
EUR 180.12

1000 ug/mL Fluometuron in Acetonitrile

LCS-2026 1ML
EUR 144.78

1000 ug/mL Malaoxon in Acetonitrile

LCS-2356 1ML
EUR 308.94

1000 ug/mL Metribuzin in Acetonitrile

LCS-2620 1ML
EUR 197.22

1000 ug/mL Mevinphos in Acetonitrile

LCS-2625 1ML
EUR 180.12

1000 ug/mL Monocrotophos in Acetonitrile

LCS-2645 1ML
EUR 93.48

1000 ug/mL Prophos in Acetonitrile

LCS-3185 1ML
EUR 93.48

1000 ug/mL Safrotin in Acetonitrile

LCS-3260 1ML
EUR 304.38

1000 ug/mL Baythroid in Acetonitrile

LCS-376 1ML
EUR 93.48

1000 ug/mL Prothioconazole in Methanol

LCS-4904 1ML
EUR 132.24

1000 ug/mL Toltrazuril in Methanol

LCS-5874 1ML
EUR 306.66

1000 ug/mL Flufenoxuron in Acetone

LCS-6009 1ML
EUR 308.94

1000 ug/mL Metconazole in Acetone

LCS-6010 1ML
EUR 152.76

1000 ug/mL Sulfentrazone in Acetone

LCS-6013 1ML
EUR 148.2

1000 ug/mL Iprovalicarb in Acetone

LCS-6029 1ML
EUR 158.46

1000 ug/mL Fosthiazate in Acetone

LCS-6080 1ML
EUR 391.02

1000 ug/mL Tetraconazole in Acetone

LCS-6089 1ML
EUR 306.66

1000 ug/mL Piperophos in Acetonitrile

LCS-6099 1ML
EUR 285

1000 ug/mL Nitenpyram in Acetonitrile

LCS-6187 1ML
EUR 197.22

4-Aminoazotoluene 1000ug/mL in MeOH

EUR 101.46

4-Aminoazotoluene 2000ug/mL in MeOH

EUR 145.92

4-Aminobiphenyl 1000ug/mL in MeOH

EUR 57

Kvantifikace mRNA pomocí reverzní transkripční PCR v reálném čase (RT-PCR): stylish a problémy.

 The fluorescence-based real-time reverse transcription PCR (RT-PCR) is broadly used for the quantification of steady-state mRNA ranges and is a vital instrument for primary analysis, molecular drugs and biotechnology. Assays are simple to carry out, able to excessive throughput, and may mix excessive sensitivity with dependable specificity. The know-how is evolving quickly with the introduction of latest enzymes, chemistries and instrumentation.

Nonetheless, whereas real-time RT-PCR addresses many of the difficulties inherent in typical RT-PCR, it has develop into more and more clear that it engenders new issues that require pressing consideration. Due to this fact, along with offering a snapshot of the state-of-the-art in real-time RT-PCR, this evaluation has an extra intention: it’ll describe and talk about critically a number of the issues related to deciphering outcomes which can be numerical and lend themselves to statistical evaluation, but whose accuracy is considerably affected by reagent and operator variability.

Bacillus thuringiensis a jeho pesticidní krystalické proteiny.

 Throughout the previous decade the pesticidal bacterium Bacillus thuringiensis has been the topic of intensive analysis. These efforts have yielded appreciable information in regards to the advanced relationships between the construction, mechanism of motion, and genetics of the organism’s pesticidal crystal proteins, and a coherent image of those relationships is starting to emerge.

Different research have centered on the ecological function of the B. thuringiensis crystal proteins, their efficiency in agricultural and different pure settings, and the evolution of resistance mechanisms in goal pests. Armed with this data base and with the instruments of contemporary biotechnology, researchers at the moment are reporting promising leads to engineering more-useful toxins and formulations, in creating transgenic vegetation that categorical pesticidal exercise, and in setting up built-in administration methods to insure that these merchandise are utilized with most effectivity and profit.

NCBI GEO: těžba desítek milionů profilů výrazů – aktualizace databáze a nástrojů.

 The Gene Expression Omnibus (GEO) repository on the Nationwide Heart for Biotechnology Info (NCBI) archives and freely disseminates microarray and different types of high-throughput information generated by the scientific neighborhood. The database has a minimal details about a microarray experiment (MIAME)-compliant infrastructure that captures absolutely annotated uncooked and processed information. A number of information deposit choices and codecs are supported, together with internet varieties, spreadsheets, XML and Easy Omnibus Format in Textual content (SOFT).

Along with information storage, a group of user-friendly web-based interfaces and functions can be found to assist customers successfully discover, visualize and obtain the 1000’s of experiments and tens of hundreds of thousands of gene expression patterns saved in GEO. This paper gives a abstract of the GEO database construction and person services, and describes latest enhancements to database design, efficiency, submission format choices, information question and retrieval utilities. GEO is accessible at http://www.ncbi.nlm.nih.gov/geo/

Glide: nový přístup professional rychlé a přesné dokování a skórování. 2. Faktory obohacení při screeningu databáze.

  • Glide’s capability to determine lively compounds in a database display is characterised by making use of Glide to a various set of 9 protein receptors. In lots of instances, two, and even three, protein websites are employed to probe the sensitivity of the outcomes to the location geometry.
  • To make the database screens as lifelike as doable, the screens use units of “druglike” decoy ligands which have been chosen to be consultant of what we consider is prone to be discovered within the compound assortment of a pharmaceutical or biotechnology firm. Outcomes are introduced for releases 1.8, 2.0, and a couple of.5 of Glide. The comparisons present that common measures for each “early” and “world” enrichment for Glide 2.5 are three instances increased than for Glide 1.Eight and greater than 2 instances increased than for Glide 2.Zero due to higher outcomes for the least well-handled screens.
  • This enchancment in enrichment stems largely from the higher steadiness of the extra broadly parametrized GlideScore 2.5 perform and the inclusion of phrases that penalize ligand-protein interactions that violate established ideas of bodily chemistry, significantly because it issues the publicity to solvent of charged protein and ligand teams.
  • Comparisons to outcomes for the thymidine kinase and estrogen receptors printed by Rognan and associates (J. Med. Chem. 2000, 43, 4759-4767) present that Glide 2.5 performs higher than GOLD 1.1, FlexX 1.8, or DOCK 4.01.

Jednoduchá šifra řídí rozpoznávání DNA pomocí TAL efektorů.

 TAL effectors of plant pathogenic micro organism within the genus Xanthomonas bind host DNA and activate genes that contribute to illness or activate protection. Goal specificity will depend on an effector-variable variety of usually 34 amino acid repeats, however the mechanism of recognition shouldn’t be understood.

We present {that a} repeat-variable pair of residues specifies the nucleotides within the goal web site, one pair to 1 nucleotide, with no obvious context dependence. Our discovering represents a beforehand unknown mechanism for protein-DNA recognition that explains TAL effector specificity, permits goal web site prediction, and opens prospects to be used of TAL effectors in analysis and biotechnology.

Referenční sekvence NCBI (RefSeq): aktuální stav, nové funkce a zásady anotací genomu.

 The Nationwide Heart for Biotechnology Info (NCBI) Reference Sequence (RefSeq) database is a group of genomic, transcript and protein sequence information. These information are chosen and curated from public sequence archives and characterize a major discount in redundancy in comparison with the quantity of information archived by the Worldwide Nucleotide Sequence Database Collaboration. The database consists of over 16,00 organisms, 2.4 × 0(6) genomic information, 13 × 10(6) proteins and a couple of × 10(6) RNA information spanning prokaryotes, eukaryotes and viruses (RefSeq launch 49, September 2011).

The RefSeq database is maintained by a mixed strategy of automated analyses, collaboration and handbook curation to generate an up-to-date illustration of the sequence, its options, names and cross-links to associated sources of data. We report right here on latest development, the standing of curating the human RefSeq information set, extra in depth characteristic annotation and present coverage for eukaryotic genome annotation by way of the NCBI annotation pipeline.

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