NCBI Reference Sequence (RefSeq):

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• 10 května, 202129 dubna, 2021
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NCBI Reference Sequence (RefSeq): upravená neredundantní databáze sekvencí genomů, transkriptů a proteinů.

 The Nationwide Heart for Biotechnology Info (NCBI) Reference Sequence (RefSeq) database (http://www.ncbi.nlm.nih.gov/RefSeq/) gives a non-redundant assortment of sequences representing genomic information, transcripts and proteins. Though the objective is to supply a complete dataset representing the whole sequence data for any given species, the database pragmatically consists of sequence information which can be presently publicly accessible within the archival databases. The database incorporates information from over 2400 organisms and consists of over a million proteins representing vital taxonomic variety spanning prokaryotes, eukaryotes and viruses.

Nucleotide and protein sequences are explicitly linked, and the sequences are linked to different assets together with the NCBI Map Viewer and Gene. Sequences are annotated to incorporate coding areas, conserved domains, variation, references, names, database cross-references, and different options utilizing a mixed strategy of collaboration and different enter from the scientific neighborhood, automated annotation, propagation from GenBank and curation by NCBI employees.

FluoroQuest™ Mounting Medium with DAPI
20004	AAT Bioquest	50 mL	EUR 109

DAPI
B1098-25	Biovision	each	EUR 496.8

DAPI
B1098-5	Biovision	each	EUR 170.4

DAPI
abx082063-10mg	Abbexa	10 mg	EUR 276

FluoroQuestâ„¢ Anti-fading Mounting Medium with DAPI
20005-20mL	AAT Bioquest	20 mL	EUR 211
Description: FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

FluoroQuest™ Anti-fading Mounting Medium with DAPI
20005	AAT Bioquest	20 mL	EUR 211

DAPI Dilactate
17509	AAT Bioquest	25 mg	EUR 109

DAPI Dilactate
17509-25mg	AAT Bioquest	25 mg	EUR 109
Description: DAPI is a fluorescent stain that binds strongly to DNA.

DAPI (hydrochloride)
C3362-10	ApexBio	10 mg	EUR 146.4

DAPI (hydrochloride)
C3362-25	ApexBio	25 mg	EUR 240

DAPI (hydrochloride)
C3362-5	ApexBio	5 mg	EUR 111.6

DAPI (dihydrochloride)
HY-D0814	MedChemExpress	50mg	EUR 439.2

DAPI Staining Kit
abx097206-1Kit	Abbexa	1 Kit	EUR 276

DAPI (Molecular Biology Grade)
CE117	GeneOn	5 mg	EUR 72

DAPI (Molecular Biology Grade)
CE118	GeneOn	25 mg	EUR 159.6

DAPI (Molecular Biology Grade)
CE119	GeneOn	100 mg	EUR 382.8

Anti-Fade Mounting Medium (With DAPI)
abx090690-10ml	Abbexa	10 ml	EUR 243.6

EverBrite Hardset Mounting Medium with DAPI
23004	Biotium	10mL	EUR 255.6
Description: Minimum order quantity: 1 unit of 10mL

DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride)
B551	ABM	0.2 ml	EUR 129.6

Fluazifop solution 10 µg/ml in ethyl acetate
GW2901-2	Glentham Life Sciences	2	EUR 126.2

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *10 mM solution in water*
17507	AAT Bioquest	2 mL	EUR 86

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *10 mM solution in water*
17507-2mL	AAT Bioquest	2 mL	EUR 86
Description: DAPI is a fluorescent stain that binds strongly to DNA.

1000 ug/mL Fluorenone in Methanol
S-4332	Scientific Laboratory Supplies	1ML	EUR 114

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*
17510	AAT Bioquest	10 mg	EUR 86

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*
17511	AAT Bioquest	100 mg	EUR 222

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*
17513	AAT Bioquest	25 mg	EUR 109

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*
17510-10mg	AAT Bioquest	10 mg	EUR 86
Description: DAPI is a fluorescent stain that binds strongly to DNA.

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*
17511-100mg	AAT Bioquest	100 mg	EUR 222
Description: DAPI is a fluorescent stain that binds strongly to DNA.

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*
17513-25mg	AAT Bioquest	25 mg	EUR 109
Description: DAPI is a fluorescent stain that binds strongly to DNA.

PAH Std Fluorene 2000ug/mL in Toluene
REPAH106	Scientific Laboratory Supplies	1ML	EUR 58.36

PAH Std Fluoranthene 1000ug/mL in DCM
REPAH158	Scientific Laboratory Supplies	1ML	EUR 59.67

PAH Std Fluoranthene 2000ug/mL in DCM
REPAH159	Scientific Laboratory Supplies	1ML	EUR 73.11

Human 4’,6-Diamidino-2-phenylindole dihydrochloride（DAPI)ELISA kit
QY-E05538	Qayee Biotechnology	96T	EUR 433.2

PAH Std Fluoroanthene 2000ug/mL in Toluene
REPAH105	Scientific Laboratory Supplies	1ML	EUR 58.36

DiagPoly Plain Fluorescent Polystyrene Particles, Pink, 0.1-0.3 µm
DNM-L012	Creative Diagnostics	2 mL	EUR 788.4

DiagPoly Plain Fluorescent Polystyrene Particles, Yellow, 0.1-0.3 µm
DNM-L002	Creative Diagnostics	2 mL	EUR 788.4

DiagPoly Plain Fluorescent Polystyrene Particles, Purple, 0.1-0.3 µm
DNM-L015	Creative Diagnostics	2 mL	EUR 788.4

DiagPoly Amine Fluorescent Polystyrene Particles, Yellow, 0.09-0.3 µm
DNM-L056	Creative Diagnostics	2 mL	EUR 832.8

DiagPoly Carboxyl Fluorescent Polystyrene Particles, Yellow, 0.09-0.3 µm
DNM-L063	Creative Diagnostics	2 mL	EUR 789.6

2-Fluorodiphenyl Ether 50ug/mL in Isooctane
REPBDE0300	Scientific Laboratory Supplies	1ML	EUR 540.52

4-Fluorodiphenyl Ether 50ug/mL in Isooctane
REPBDE0301	Scientific Laboratory Supplies	1ML	EUR 540.52

PAH Std 9-Fluorobenzo(k)fluoranthene 100ug/mL in Toluene
REPAH9016-I	Scientific Laboratory Supplies	1ML	EUR 1373.49

VOC Int Std Fluorobenzene 2000ug/mL in MeOH
REVOC001-I	Scientific Laboratory Supplies	1ML	EUR 160.59

VOC Int Std Fluorobenzene 1000ug/mL in MeOH
REVOC002-I	Scientific Laboratory Supplies	1ML	EUR 124.03

VOC Int Std Fluorobenzene 4000ug/mL in MeOH
REVOC003-I	Scientific Laboratory Supplies	1ML	EUR 62.54

PAH Std 1-Fluoropyrene 100ug/mL in Toluene
REPAH9012-I	Scientific Laboratory Supplies	1ML	EUR 365.57

DiagPoly Plain Fluorescent Polystyrene Particles, Nile Red, 0.1-0.3 µm
DNM-L009	Creative Diagnostics	2 mL	EUR 788.4

DiagPoly Plain Fluorescent Polystyrene Particles, Sky Blue, 0.1-0.3 µm
DNM-L019	Creative Diagnostics	2 mL	EUR 788.4

DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) for nucleic acid staining
AR1176	BosterBio	10mL(100 assys)	EUR 78

DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) for nucleic acid staining
AR1177	BosterBio	50mL(500 assys)	EUR 121.2

PAH Std 2-Fluorofluorene 100ug/mL in Toluene
REPAH9004-I	Scientific Laboratory Supplies	1ML	EUR 130.56

PAH Std 1-Fluorochrysene 100ug/mL in Toluene
REPAH9013-I	Scientific Laboratory Supplies	1ML	EUR 641.05

PAH Std 3-Fluorochrysene 100ug/mL in Toluene
REPAH9014-I	Scientific Laboratory Supplies	1ML	EUR 318.57

DiagPoly Plain Fluorescent Polystyrene Particles, Light Yellow, 0.1-0.3 µm
DNM-L005	Creative Diagnostics	2 mL	EUR 788.4

DiagPoly Carboxyl Fluorescent Polystyrene Particles, Nile Red, 0.09-0.3 µm
DNM-L071	Creative Diagnostics	2 mL	EUR 788.4

PAH Std 2-Fluorophenanthrene 100ug/mL in Toluene
REPAH9007-I	Scientific Laboratory Supplies	1ML	EUR 318.57

PAH Std 3-Fluorophenanthrene 100ug/mL in Toluene
REPAH9008-I	Scientific Laboratory Supplies	1ML	EUR 318.57

PAH Std 4-Fluorophenanthrene 100ug/mL in Toluene
REPAH9009-I	Scientific Laboratory Supplies	1ML	EUR 494.82

PAH Std 3-Fluorofluoranthene 100ug/mL in Toluene
REPAH9011-I	Scientific Laboratory Supplies	1ML	EUR 130.56

PAH Std Benzo(b)fluoranthene 2000ug/ml in MeOH
REPAH145	Scientific Laboratory Supplies	1ML	EUR 160.59

PAH Std 5-Fluoroacenaphthylene 100ug/mL in Toluene
REPAH9002-I	Scientific Laboratory Supplies	1ML	EUR 445.21

PAH Std 4-Fluorodiphenylmethane 100ug/mL in Toluene
REPAH9003-I	Scientific Laboratory Supplies	1ML	EUR 168.42

PAH Std 2-Fluorodiphenylmethane 100ug/mL in Toluene
REPAH9005-I	Scientific Laboratory Supplies	1ML	EUR 168.42

PAH Std 2-Fluorodibenzothiophene 100ug/mL in Toluene
REPAH7003	Scientific Laboratory Supplies	1ML	EUR 983.12

PAH Std Benzo(k)fluoranthene 2000ug/ml in Toluene
REPAH1141	Scientific Laboratory Supplies	1ML	EUR 147.53

PAH Std Benzo(a)fluoranthene 200ug/mL in Toluene
REPAH2401	Scientific Laboratory Supplies	1ML	EUR 1143.71

PAH Std Benzo(k)fluoroanthene 2000ug/mL in Toluene
REPAH114	Scientific Laboratory Supplies	1ML	EUR 59.67

PAH Std Benzo(ghi)fluoranthene 200ug/mL in Toluene
REPAH2402	Scientific Laboratory Supplies	1ML	EUR 1423.1

PAH Std Dibenzo(ae)fluoranthene 200ug/mL in Toluene
REPAH2502	Scientific Laboratory Supplies	1ML	EUR 1869.62

PAH Std 7H-Benzo(c)fluorene 200ug/mL in Toluene
REPAH4104	Scientific Laboratory Supplies	1ML	EUR 1065.37

50 ML AMPHOTERICIN B, LIQUID 250 µG/ML SOLUBILIZED
30-003-CF	CORNING	50 mL/pk	EUR 97.2
Description: Media Catalog; Cell Culture Reagents

50 ML KANAMYCIN SULFATE, LIQUID, 5,000 µG/ML SOLUTION
30-006-CF	CORNING	50 mL/pk	EUR 117.6
Description: Media Catalog; Cell Culture Reagents

PAH Std 11H-Benzo(a)fluorene 1000ug/mL in Toluene
REPAH4101	Scientific Laboratory Supplies	1ML	EUR 736.36

PAH Std 11H-Benzo(b)fluorene 200ug/mL in Toluene
REPAH4103	Scientific Laboratory Supplies	1ML	EUR 485.68

PAH Std Benzo(b)fluoranthene-d12 100ug/mL in Toluene
REPAH1104	Scientific Laboratory Supplies	1ML	EUR 365.57

PAH Std Benzo(k)fluoranthene-d12 100ug/mL in Toluene
REPAH1105	Scientific Laboratory Supplies	1ML	EUR 365.57

PAH Std Indeno(123-cd)fluoranthene 200ug/mL in Toluene
REPAH2506	Scientific Laboratory Supplies	1ML	EUR 1423.1

PAH Std Naphtho(12-b)fluoranthene 200ug/mL in Toluene
REPAH2601	Scientific Laboratory Supplies	1ML	EUR 3719.65

PAH Std Naphtho(12-k)fluoranthene 200ug/mL in Toluene
REPAH2602	Scientific Laboratory Supplies	1ML	EUR 1423.1

PAH Std Naphtho(23-b)fluoranthene 200ug/mL in Toluene
REPAH2603	Scientific Laboratory Supplies	1ML	EUR 2417.97

PAH Std Naphtho(23-j)fluoranthene 200ug/mL in Toluene
REPAH2604	Scientific Laboratory Supplies	1ML	EUR 3719.65

PAH Std Naphtho(23-k)fluoranthene 200ug/mL in Toluene
REPAH2605	Scientific Laboratory Supplies	1ML	EUR 987.03

3-Fluoro-24-dibromodiphenyl Ether 50ug/mL in Isooctane
REPBDE0305	Scientific Laboratory Supplies	1ML	EUR 1014.45

3-Fluoro-34-dibromodiphenyl Ether 50ug/mL in Isooctane
REPBDE0306	Scientific Laboratory Supplies	1ML	EUR 1014.45

2-Fluoro-244-tribromodiphenyl Ether 50ug/mL in Toluene
REPBDE0309	Scientific Laboratory Supplies	1ML	EUR 1014.45

Sodium Fluoride, 1 M, 100 ML
M-CSS-290	MiTeGen	100 ml	EUR 94
Description: Sodium Fluoride, 1 M, 100 ML

4-Fluoro-234-tribromodiphenyl Ether 50ug/mL in Isooctane
REPBDE0307	Scientific Laboratory Supplies	1ML	EUR 1321.27

4-Fluoro-236-tribromodiphenyl Ether 50ug/mL in Isooctane
REPBDE0308	Scientific Laboratory Supplies	1ML	EUR 836.89

2-Fluoro-244-tribromodiphenyl Ether 50ug/mL in Isooctane
REPBDE0310	Scientific Laboratory Supplies	1ML	EUR 1014.45

Fluorescent colour microtube 0.5 mL
DD45423	Scientific Laboratory Supplies	PK1000	EUR 99.18

6-Fluoro-2244-tetrabromodiphenyl Ether 50ug/mL in Toluene
REPBDE0311	Scientific Laboratory Supplies	1ML	EUR 1047.09

Ammonium Fluoride, 10 M, 100 ML
M-CSS-134	MiTeGen	4 x 25 ml	EUR 120
Description: Ammonium Fluoride, 10 M, 100 ML

3-Fluoro-22446-pentabromodiphenyl Ether 50ug/mL in Toluene
REPBDE0319	Scientific Laboratory Supplies	1ML	EUR 1463.58

4-Fluoro-233456-hexabromodiphenyl Ether 50ug/mL in Toluene
REPBDE0323	Scientific Laboratory Supplies	1ML	EUR 2009.32

6-Fluoro-2244-tetrabromodiphenyl Ether 50ug/mL in Isooctane
REPBDE0312	Scientific Laboratory Supplies	1ML	EUR 1047.09

6-Fluoro-2344-tetrabromodiphenyl Ether 50ug/mL in Isooctane
REPBDE0315	Scientific Laboratory Supplies	1ML	EUR 1342.16

4-Fluoro-2346-tetrabromodiphenyl Ether 50ug/mL in Isooctane
REPBDE0316	Scientific Laboratory Supplies	1ML	EUR 1342.16

3-Fluoro-22446-pentabromodiphenyl Ether 50ug/mL in Isooctane
REPBDE0320	Scientific Laboratory Supplies	1ML	EUR 1463.58

3-Fluoro-23446-pentabromodiphenyl Ether 50ug/mL in Isooctane
REPBDE0321	Scientific Laboratory Supplies	1ML	EUR 1297.77

3-Fluoro-2244556-heptabromodiphenyl Ether 50ug/mL in Toluene
REPBDE0324	Scientific Laboratory Supplies	1ML	EUR 2009.32

4-Fluoro-223345566-nonabromodiphenyl Ether 50ug/mL in Toluene
REPBDE0327	Scientific Laboratory Supplies	1ML	EUR 1383.94

PAH Std 9-Fluoro-5-methylchrysene 50ug/mL in Isooctane
REPAH9015-I	Scientific Laboratory Supplies	1ML	EUR 1245.54

4-Fluoro-223345566-nonabromodiphenyl Ether 50ug/mL in Isooctane
REPBDE0326	Scientific Laboratory Supplies	1ML	EUR 1383.94

PAH Std 2-Fluoro-6-methylnaphthalene 100ug/mL in Isooctane
REPAH9001-I	Scientific Laboratory Supplies	1ML	EUR 73.11

PAH Std 3-Fluoro-6-methylphenanthrene 50ug/mL in Isooctane
REPAH9010-I	Scientific Laboratory Supplies	1ML	EUR 1218.12

Amphotericin B (Fungizone) Sol, 250 µg/mL
CCM1120-100	Bio Basic	100 mL	EUR 132.28

PAH Std 5-Fluoro-23-dimethylbenzothiophene 100ug/mL in Isooctane
REPAH7002	Scientific Laboratory Supplies	1ML	EUR 983.12

Latex-in-a-Box Conjugation Kit, Blue 0.3 micron, 7 mL
LIB03-B007	BioAssayWorks	7 mL	EUR 880.73
Description: Latex-in-a-Box Conjugation Kit conjugates blue 0.3 micron latex nanoparticles to antibodies and proteins. The nanoparticles are created using a special technology that needs only one hour to conjugate the particles to the antibody/protein.

Latex-in-a-Box Conjugation Kit, Red 0.3 micron, 7 mL
LIB03-R007	BioAssayWorks	7 mL	EUR 880.73
Description: Latex-in-a-Box Conjugation Kit conjugates red 0.3 micron latex nanoparticles to antibodies and proteins. The nanoparticles are created using a special technology that needs only one hour to conjugate the particles to the antibody/protein.

Lasalocid A sodium salt Solution, ~100 µg/ml in acetonitrile
GW0266-1	Glentham Life Sciences	1	EUR 126.2

Lasalocid A sodium salt Solution, ~100 µg/ml in acetonitrile
GW0266-2500	Glentham Life Sciences	2500	EUR 196.1

1000 ug/mL Fluometuron in Acetonitrile
LCS-2026	Scientific Laboratory Supplies	1ML	EUR 174

VOC Int Std 2-Bromo-1Chloropropane Fluorobenzene 2000ug/mL in MeOH
REVOC007-I	Scientific Laboratory Supplies	1ML	EUR 110.98

IC Anion Std Mono Fluorophosphate 1mg/mL
ICAS37	Scientific Laboratory Supplies	100ML	EUR 122.73

PAH Std 5-Fluoro-3-methylbenzo(b)thiophene 100ug/mL in Isooctane
REPAH7001	Scientific Laboratory Supplies	1ML	EUR 201.06

VOC Int Std 2-Bromo-1-Chloropropane Fluorobenzene 1000ug/mL in MeOH
REVOC005-I	Scientific Laboratory Supplies	1ML	EUR 110.98

1000 ug/mL Fluoxastrobin in HPLC Acetonitrile
LCS-6056	Scientific Laboratory Supplies	1ML	EUR 354

1000 ug/mL Fluoxastrobin in HPLC Acetonitrile
S-6020	Scientific Laboratory Supplies	1ML	EUR 134.4

Penicillin-Streptomycin 100X Solution, 10, 000 Units/mL, 10, 000 µg/mL
CCM1133-100	Bio Basic	100 mL	EUR 76.86

Starch Indicator 0.3%
ST0305	Scientific Laboratory Supplies	500ML	EUR 117.5

VOC 4-BFB 12-Dichlorobenzene D4 Fluorobenz 1000ug/mL MeOH
REVOC003-FS	Scientific Laboratory Supplies	1ML	EUR 59.8

100 ML PENICILLIN-STREPTOMYCIN-L-GLUTAMINE, 100X, 10,000 I.U. PENCILLIN (PER ML) 10,000 µG/ML STREPTOMYCIN 29.2 MG/ML L-GLUTAMINE
30-009-CI	CORNING	100 mL/pk	EUR 96
Description: Media Catalog; Cell Culture Reagents

1000 ug/mL Flufenoxuron in Acetone
LCS-6009	Scientific Laboratory Supplies	1ML	EUR 375.6

1000 ug/mL Flufenacet in Acetonitrile
S-4906-ACN	Scientific Laboratory Supplies	1ML	EUR 159.6

1000 ug/mL Flubenzimin in Acetonitrile
S-6385	Scientific Laboratory Supplies	1ML	EUR 211.2

Human Paraoxonase 1 (PON) Standards QQ and RR Phenotypes (2 x 0.3 mL)
0801384	Zeptometrix	2 x 0.3 mL	EUR 339

Human Paraoxonase 1 (PON) Standards QQ and RR Phenotypes (2 x 0.3 mL)
801384	Zeptometrix	2 x 0.3 mL	EUR 323

1000 ug/mL Flufenacet in HPLC Acetonitrile
LCS-4906	Scientific Laboratory Supplies	1ML	EUR 356.4

1000 ug/mL Flufenoxuron in HPLC Acetonitrile
S-6009	Scientific Laboratory Supplies	1ML	EUR 250.8

Brilliant Cresyl Blue (0.3%, Alcoholic)
BCA3800	ScyTek Laboratories	1 Gal.	EUR 289.2

Brilliant Cresyl Blue (0.3%, Alcoholic)
BCA500	ScyTek Laboratories	500 ml	EUR 108

Brilliant Cresyl Blue (0.3%, Alcoholic)
BCA999	ScyTek Laboratories	1000 ml	EUR 139.2

Hydrogen Peroxide 0.3% w/v
HP035	Scientific Laboratory Supplies	5L	EUR 151.2

Hydrogen Peroxide 0.3% w/v
HP03F	Scientific Laboratory Supplies	1L	EUR 54

Vanadium Foil 0.3 mm, 99.9%
GX3211-100	Glentham Life Sciences	100	EUR 572.1

IC Anion Std Fluoride 1000ppm in H2O
ICAB03	Scientific Laboratory Supplies	500ML	EUR 121.2

IC Anion Std Fluoride 1000ppm in H2O
ICAS03-B	Scientific Laboratory Supplies	250ML	EUR 177.6

IC Anion Std Fluoride 500ppm in H2O
ICAT411	Scientific Laboratory Supplies	1L	EUR 216.73

IC Anion Std Fluoride 100ppm in H2O
ICAU03	Scientific Laboratory Supplies	100ML	EUR 72

IC Anion Std Fluoride 5000mg/L in H2O
ICAE03	Scientific Laboratory Supplies	500ML	EUR 182.78

IC Anion Std Fluoride 10000mg/L in H2O
ICAS0310M	Scientific Laboratory Supplies	500ML	EUR 285.6

IC Anion Std Fluoride 500mg/L in H2O
ICAT4105	Scientific Laboratory Supplies	500ML	EUR 116.4

0.3 ml 8-Strip Tubes & Domed Caps, 250 Strips of 8 Tubes and Caps
GP209	GenDepot	2,000 tubes/caps	EUR 400.8

Random Hexamers 100 µg
26-4000-03	Gene Link	100 ug	EUR 150
Description: Random Primers are a mixture of oligonucleotides representing all possible sequence for that size. Random Primers can be used to prime synthesis in oligo-labeling similar to using hexamers (1,2) and cDNA synthesis. Random prime labeling yields high specific activity labeled DNA probe which can be used for all southern, northern and in situ hybridization studies. Random Primers can be also used similar to using hexamers in cDNA synthesis in combination with oligo d(T) to yield more 5' end cDNA sequence. Recently random primers have been used to detect DNA polymorphism. These polymorphisms, simply detected as DNA segments which amplify from one parent but not the other, are inherited in a Mendelian fashion and can be used to construct genetic maps in a variety of species. The authors suggested that these polymorphisms be called RAPD (pronounced RAPID) makers, after Random Amplified Polymorphic DNA (3). References 1. Feinberg, A.P. & Vogelstein, B. (1983) Anal. Biochem. 132:6-13. 2. Feinberg, A.P. & Vogelstein, B. (1984) Anal. Biochem. 137:266-267. 3. Williams J. G., Kubelik A.R., Livak K.J., Rafalski J.A. & Tingey S.V. (1990) Nucleic Acid Res. 18(22):6531-5.

1000 ug/mL Flubendiamide in HPLC Grade Acetonitrile
LCS-5773-ACN	Scientific Laboratory Supplies	1ML	EUR 106.8

DiagPoly Amine Polystyrene Particles, 0.3 µm
DNM-F002	Creative Diagnostics	10 mL	EUR 696

Molybdenum Wire 0.3 mm diameter, 99.95%
GX7025-100	Glentham Life Sciences	100	EUR 228.1

Molybdenum Wire 0.3 mm diameter, 99.95%
GX7025-25	Glentham Life Sciences	25	EUR 111.5

Gold Wire 0.3 mm diameter, 99.9%
GX7550-1	Glentham Life Sciences	1	EUR 400.9

Palladium Wire 0.3 mm diameter, 99.9%
GX8261-1	Glentham Life Sciences	1	EUR 182.5

Tungsten Wire 0.3 mm diameter, 99.95+%
GX2248-100	Glentham Life Sciences	100	EUR 234.6

Tungsten Wire 0.3 mm diameter, 99.95+%
GX2248-25	Glentham Life Sciences	25	EUR 110

Gold Powder 0.3-3 micron, 99.9+%
GX1014-1	Glentham Life Sciences	1	EUR 226.6

Gold Powder 0.3-3 micron, 99.9+%
GX1014-5	Glentham Life Sciences	5	EUR 1098.8

Platinum Wire 0.3 mm diameter, 99.9%
GX1042-1	Glentham Life Sciences	1	EUR 353.4

Platinum Wire 0.3 mm diameter, 99.9%
GX1042-25	Glentham Life Sciences	25	EUR 134.1

Platinum Wire 0.3 mm diameter, 99.99%
GX1234-1	Glentham Life Sciences	1	EUR 366.5

Platinum Wire 0.3 mm diameter, 99.99%
GX1234-25	Glentham Life Sciences	25	EUR 175.9

Pesticide Std Fluchloralin 1000ug/mL in MeOH
REPET360	Scientific Laboratory Supplies	1ML	EUR 96.61

Pesticide Std Fluchloralin 2000ug/mL in MeOH
REPET361	Scientific Laboratory Supplies	1ML	EUR 110.98

Kvantifikace mRNA pomocí reverzní transkripční PCR v reálném čase (RT-PCR): stylish a problémy.

 The fluorescence-based real-time reverse transcription PCR (RT-PCR) is broadly used for the quantification of steady-state mRNA ranges and is a vital instrument for primary analysis, molecular drugs and biotechnology. Assays are simple to carry out, able to excessive throughput, and may mix excessive sensitivity with dependable specificity. The know-how is evolving quickly with the introduction of latest enzymes, chemistries and instrumentation.

Nonetheless, whereas real-time RT-PCR addresses many of the difficulties inherent in typical RT-PCR, it has develop into more and more clear that it engenders new issues that require pressing consideration. Due to this fact, along with offering a snapshot of the state-of-the-art in real-time RT-PCR, this evaluation has an extra intention: it’ll describe and talk about critically a number of the issues related to deciphering outcomes which can be numerical and lend themselves to statistical evaluation, but whose accuracy is considerably affected by reagent and operator variability.

Bacillus thuringiensis a jeho pesticidní krystalické proteiny.

 Throughout the previous decade the pesticidal bacterium Bacillus thuringiensis has been the topic of intensive analysis. These efforts have yielded appreciable information in regards to the advanced relationships between the construction, mechanism of motion, and genetics of the organism’s pesticidal crystal proteins, and a coherent image of those relationships is starting to emerge.

Different research have centered on the ecological function of the B. thuringiensis crystal proteins, their efficiency in agricultural and different pure settings, and the evolution of resistance mechanisms in goal pests. Armed with this data base and with the instruments of contemporary biotechnology, researchers at the moment are reporting promising leads to engineering more-useful toxins and formulations, in creating transgenic vegetation that categorical pesticidal exercise, and in setting up built-in administration methods to insure that these merchandise are utilized with most effectivity and profit.

NCBI GEO: těžba desítek milionů profilů výrazů – aktualizace databáze a nástrojů.

 The Gene Expression Omnibus (GEO) repository on the Nationwide Heart for Biotechnology Info (NCBI) archives and freely disseminates microarray and different types of high-throughput information generated by the scientific neighborhood. The database has a minimal details about a microarray experiment (MIAME)-compliant infrastructure that captures absolutely annotated uncooked and processed information. A number of information deposit choices and codecs are supported, together with internet varieties, spreadsheets, XML and Easy Omnibus Format in Textual content (SOFT).

Along with information storage, a group of user-friendly web-based interfaces and functions can be found to assist customers successfully discover, visualize and obtain the 1000’s of experiments and tens of hundreds of thousands of gene expression patterns saved in GEO. This paper gives a abstract of the GEO database construction and person services, and describes latest enhancements to database design, efficiency, submission format choices, information question and retrieval utilities. GEO is accessible at http://www.ncbi.nlm.nih.gov/geo/

Glide: nový přístup professional rychlé a přesné dokování a skórování. 2. Faktory obohacení při screeningu databáze.

• Glide’s capability to determine lively compounds in a database display is characterised by making use of Glide to a various set of 9 protein receptors. In lots of instances, two, and even three, protein websites are employed to probe the sensitivity of the outcomes to the location geometry.
• To make the database screens as lifelike as doable, the screens use units of “druglike” decoy ligands which have been chosen to be consultant of what we consider is prone to be discovered within the compound assortment of a pharmaceutical or biotechnology firm. Outcomes are introduced for releases 1.8, 2.0, and a couple of.5 of Glide. The comparisons present that common measures for each “early” and “world” enrichment for Glide 2.5 are three instances increased than for Glide 1.Eight and greater than 2 instances increased than for Glide 2.Zero due to higher outcomes for the least well-handled screens.
• This enchancment in enrichment stems largely from the higher steadiness of the extra broadly parametrized GlideScore 2.5 perform and the inclusion of phrases that penalize ligand-protein interactions that violate established ideas of bodily chemistry, significantly because it issues the publicity to solvent of charged protein and ligand teams.
• Comparisons to outcomes for the thymidine kinase and estrogen receptors printed by Rognan and associates (J. Med. Chem. 2000, 43, 4759-4767) present that Glide 2.5 performs higher than GOLD 1.1, FlexX 1.8, or DOCK 4.01.

Jednoduchá šifra řídí rozpoznávání DNA pomocí TAL efektorů.

 TAL effectors of plant pathogenic micro organism within the genus Xanthomonas bind host DNA and activate genes that contribute to illness or activate protection. Goal specificity will depend on an effector-variable variety of usually 34 amino acid repeats, however the mechanism of recognition shouldn’t be understood.

We present {that a} repeat-variable pair of residues specifies the nucleotides within the goal web site, one pair to 1 nucleotide, with no obvious context dependence. Our discovering represents a beforehand unknown mechanism for protein-DNA recognition that explains TAL effector specificity, permits goal web site prediction, and opens prospects to be used of TAL effectors in analysis and biotechnology.

Referenční sekvence NCBI (RefSeq): aktuální stav, nové funkce a zásady anotací genomu.

 The Nationwide Heart for Biotechnology Info (NCBI) Reference Sequence (RefSeq) database is a group of genomic, transcript and protein sequence information. These information are chosen and curated from public sequence archives and characterize a major discount in redundancy in comparison with the quantity of information archived by the Worldwide Nucleotide Sequence Database Collaboration. The database consists of over 16,00 organisms, 2.4 × 0(6) genomic information, 13 × 10(6) proteins and a couple of × 10(6) RNA information spanning prokaryotes, eukaryotes and viruses (RefSeq launch 49, September 2011).

The RefSeq database is maintained by a mixed strategy of automated analyses, collaboration and handbook curation to generate an up-to-date illustration of the sequence, its options, names and cross-links to associated sources of data. We report right here on latest development, the standing of curating the human RefSeq information set, extra in depth characteristic annotation and present coverage for eukaryotic genome annotation by way of the NCBI annotation pipeline.