• May 10, 2021
alidea

NCBI Reference Sequence (RefSeq):

NCBI Reference Sequence (RefSeq): upravená neredundantní databáze sekvencí genomů, transkriptů a proteinů.

 The Nationwide Heart for Biotechnology Info (NCBI) Reference Sequence (RefSeq) database (http://www.ncbi.nlm.nih.gov/RefSeq/) gives a non-redundant assortment of sequences representing genomic information, transcripts and proteins. Though the objective is to supply a complete dataset representing the whole sequence data for any given species, the database pragmatically consists of sequence information which can be presently publicly accessible within the archival databases. The database incorporates information from over 2400 organisms and consists of over a million proteins representing vital taxonomic variety spanning prokaryotes, eukaryotes and viruses.

Nucleotide and protein sequences are explicitly linked, and the sequences are linked to different assets together with the NCBI Map Viewer and Gene. Sequences are annotated to incorporate coding areas, conserved domains, variation, references, names, database cross-references, and different options utilizing a mixed strategy of collaboration and different enter from the scientific neighborhood, automated annotation, propagation from GenBank and curation by NCBI employees.

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DAPI

B1098-5
EUR 142

50 ML AMPHOTERICIN B, LIQUID 250 µG/ML SOLUBILIZED

30-003-CF 50 mL/pk
EUR 81
Description: Media Catalog; Cell Culture Reagents

50 ML KANAMYCIN SULFATE, LIQUID, 5,000 µG/ML SOLUTION

30-006-CF 50 mL/pk
EUR 98
Description: Media Catalog; Cell Culture Reagents

Amphotericin B (Fungizone) Sol, 250 µg/mL

CCM1120-100 100 mL
EUR 110.23

100 ML ANTIBIOTIC-ANTIMYCOTIC SOLUTION, 10,000 I.U. PENICILLIN (PER ML) 10,000 µG/ML STREPTOMYCIN 25 µG/ML AMPHOTERICIN WITH 8.5 G/L NACI

30-004-CI 100 mL/pk
EUR 103
Description: Media Catalog; Cell Culture Reagents

DAPI (dihydrochloride)

HY-D0814 50mg
EUR 366

DAPI Dilactate

17509 25 mg
EUR 132

DAPI (hydrochloride)

C3362-10 10 mg
EUR 122

DAPI (hydrochloride)

C3362-25 25 mg
EUR 200

DAPI (hydrochloride)

C3362-5 5 mg
EUR 93

Latex-in-a-Box Conjugation Kit, Blue 0.3 micron, 7 mL

LIB03-B007 7 mL
EUR 733.94
Description: Latex-in-a-Box Conjugation Kit conjugates blue 0.3 micron latex nanoparticles to antibodies and proteins. The nanoparticles are created using a special technology that needs only one hour to conjugate the particles to the antibody/protein.

Latex-in-a-Box Conjugation Kit, Red 0.3 micron, 7 mL

LIB03-R007 7 mL
EUR 733.94
Description: Latex-in-a-Box Conjugation Kit conjugates red 0.3 micron latex nanoparticles to antibodies and proteins. The nanoparticles are created using a special technology that needs only one hour to conjugate the particles to the antibody/protein.

Fluorguard Antifade Mounting Medium

F001 1 ml
EUR 86

Penicillin-Streptomycin 100X Solution, 10, 000 Units/mL, 10, 000 µg/mL

CCM1133-100 100 mL
EUR 64.05

100 ML PENICILLIN-STREPTOMYCIN SOLUTION, 50X, 5,000 I.U. PENICILLIN (PER ML) 5,000 µG/ML STREPTOMYCIN

30-001-CI 100 mL/pk
EUR 82
Description: Media Catalog; Cell Culture Reagents

DAPI Staining Kit

abx097206-1Kit 1 Kit
EUR 230

FluorLast? with DAPI

1214-20
EUR 153

100 ML PENICILLIN-STREPTOMYCIN-L-GLUTAMINE, 100X, 10,000 I.U. PENCILLIN (PER ML) 10,000 µG/ML STREPTOMYCIN 29.2 MG/ML L-GLUTAMINE

30-009-CI 100 mL/pk
EUR 80
Description: Media Catalog; Cell Culture Reagents

DAPI (Molecular Biology Grade)

CE117 5 mg
EUR 60

DAPI (Molecular Biology Grade)

CE118 25 mg
EUR 133

DAPI (Molecular Biology Grade)

CE119 100 mg
EUR 319

Human Paraoxonase 1 (PON) Standards QQ and RR Phenotypes (2 x 0.3 mL)

0801384 2 x 0.3 mL
EUR 330.8
Description: Please contact Gentaur in order to receive the datasheet of the product.

EverBrite Mounting Medium with DAPI

23002 10mL
EUR 195
Description: Minimum order quantity: 1 unit of 10mL

FluorLast? with DAPI and DABCO

1218-20
EUR 153

0.3 ml 8-Strip Tubes & Domed Caps, 250 Strips of 8 Tubes and Caps

GP209 2,000 tubes/caps
EUR 334

Brilliant Cresyl Blue (0.3%, Alcoholic)

BCA3800 1 Gal.
EUR 241

Brilliant Cresyl Blue (0.3%, Alcoholic)

BCA500 500 ml
EUR 90

Brilliant Cresyl Blue (0.3%, Alcoholic)

BCA999 1000 ml
EUR 116

Anti-Fade Mounting Medium (With DAPI)

abx090690-10ml 10 ml
EUR 203

EverBrite Hardset Mounting Medium with DAPI

23004 10mL
EUR 213
Description: Minimum order quantity: 1 unit of 10mL

FluoroQuest™ Mounting Medium with DAPI

20004 50 mL
EUR 132

Random Hexamers 100 µg

26-4000-03 100 ug
EUR 125
Description:

Random Primers are a mixture of oligonucleotides representing all possible sequence for that size. Random Primers can be used to prime synthesis in oligo-labeling similar to using hexamers (1,2) and cDNA synthesis. Random prime labeling yields high specific activity labeled DNA probe which can be used for all southern, northern and in situ hybridization studies. Random Primers can be also used similar to using hexamers in cDNA synthesis in combination with oligo d(T) to yield more 5' end cDNA sequence. Recently random primers have been used to detect DNA polymorphism. These polymorphisms, simply detected as DNA segments which amplify from one parent but not the other, are inherited in a Mendelian fashion and can be used to construct genetic maps in a variety of species. The authors suggested that these polymorphisms be called RAPD (pronounced RAPID) makers, after Random Amplified Polymorphic DNA (3).

References 1. Feinberg, A.P. & Vogelstein, B. (1983) Anal. Biochem. 132:6-13. 2. Feinberg, A.P. & Vogelstein, B. (1984) Anal. Biochem. 137:266-267. 3. Williams J. G., Kubelik A.R., Livak K.J., Rafalski J.A. & Tingey S.V. (1990) Nucleic Acid Res. 18(22):6531-5.

Rat Monoclonal Anti-Mouse CD4 Pur. Low Endotoxin (Clone YTS 191.1) (rat IgG2b)

MCD004-ML 100 ug
EUR 408

Rat Monoclonal Anti-Mouse CD25 , Pur. Low Endotoxin (Clone PC61.5.3) (rat IgG1)

MCD025-ML 100 ug
EUR 408

BrightGreen miRNA qPCR MasterMix-Low ROX

MasterMix-mL 4 x 1.25 ml for 500 reactions
EUR 151

DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride)

B551 0.2 ml
EUR 108

DiagPoly Amine Polystyrene Particles, 0.3 µm

DNM-F002 10 mL
EUR 580

DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *10 mM solution in water*

17507 2 mL
EUR 115

DiagPoly Plain Polystyrene Particles, 0.2-0.3 µm

DNM-P003 10 mL
EUR 554

Anti-PON1 Clone KRJ1 (100 µg)

0801012 100 µg
EUR 284
Description: Please contact Gentaur in order to receive the datasheet of the product.

Anti-PON1 Clone KRJ2 (100 µg)

0801014 100 µg
EUR 284
Description: Please contact Gentaur in order to receive the datasheet of the product.

bFGF-nAb Purified Protein 25 µg

ABP-NAB-BFGFAB 25 µg Ask for price

bFGF-nAb Plus Biotinylated 25 µg

ABP-NAB-BFGFPB 25 µg Ask for price

GFP-nAb Purified Protein 25 µg

ABP-NAB-GFPAB 25 µg Ask for price

GFP-nAb Plus Biotinylated 25 µg

ABP-NAB-GFPPB 25 µg Ask for price

mNeonGreen-nAb Purified Protein 25 µg

ABP-NAB-MNGAB 25 µg Ask for price

mNeonGreen-nAb Plus Biotinylated 25 µg

ABP-NAB-MNGPB 25 µg Ask for price

RFP-nAb Purified Protein 25 µg

ABP-NAB-RFPAB 25 ug Ask for price

RFP-nAb Plus Biotinylated 25 µg

ABP-NAB-RFPPB 25 µg Ask for price

DAPI, dilactate (4’, 6-diamidino-2-phenylindole, dilactate)

40009 10MG
EUR 146
Description: Minimum order quantity: 1 unit of 10MG

DiagPoly Streptavidin Coated Polystyrene Particles, 0.3-0.4 µm

DNM-C005 10 mL
EUR 676

Papain Solution In PBS, 1mg/ml

CA093-002 20ml Ask for price

Acridine Orange, 10mg/mL in water

40039 10ML
EUR 101
Description: Minimum order quantity: 1 unit of 10ML

Hoechst 33258, 10mg/mL in H2O

40044 10ML
EUR 217
Description: Minimum order quantity: 1 unit of 10ML

Hoechst 33342, 10mg/mL in H2O

40046 10ML
EUR 232
Description: Minimum order quantity: 1 unit of 10ML

Ethidium bromide, 10mg/ml in water

GT9211-10ML 10 ml
EUR 70

ML-210

HY-100003 10mM/1mL
EUR 161

ML-18

HY-101844 10mM/1mL
EUR 310

ML-030

HY-103050 100mg
EUR 918

ML-792

HY-108702 10mg
EUR 567

ML-290

HY-112606 5mg
EUR 234

ML-323

HY-17543 10mM/1mL
EUR 126

ML-098

HY-19800 10mM/1mL
EUR 156

ML-323

B1317-5.1 10 mM (in 1mL DMSO)
EUR 142
Description: IC50: ML-323 is a highly potent and selective inhibitor of USP1-UAF1 with IC50 value of 76 nM.ML-323 is an inhibitor of USP1-UAF1 with remarkable selectivity and relatively low cytotoxicity to the human cells.

ML 240

B4926-10 10 mg
EUR 270

ML 240

B4926-25 25 mg
EUR 537

ML 240

B4926-5 5 mg
EUR 171

ML 348

B4933-10 10 mg
EUR 279

ML 348

B4933-5.1 10 mM (in 1mL DMSO)
EUR 247

ML 348

B4933-50 50 mg
EUR 1014

ML 349

B4934-10 10 mg
EUR 311

ML 349

B4934-5 5 mg
EUR 206

ML 213

B5628-10 10 mg
EUR 196

ML 213

B5628-25 25 mg
EUR 376

ML 213

B5628-5 5 mg
EUR 126

ML 213

B5628-50 50 mg
EUR 661

ML 171

B5672-5.1 10 mM (in 1mL DMSO)
EUR 108

ML 171

B5672-50 50 mg
EUR 125

ML 204

B5696-10 10 mg
EUR 235

ML 204

B5696-25 25 mg
EUR 461

ML 204

B5696-5 5 mg
EUR 152

ML 204

B5696-50 50 mg
EUR 822

ML SA1

B5700-10 10 mg
EUR 226

ML SA1

B5700-50 50 mg
EUR 795

ML 221

B5702-10 10 mg
EUR 210

ML 221

B5702-100 100 mg
EUR 830

ML 221

B5702-25 25 mg
EUR 392

ML 202

B5739-10 10 mg
EUR 299

ML 190

B5741-10 10 mg
EUR 383

ML 190

B5741-50 50 mg
EUR 1455

ML 281

B5750-10 10 mg
EUR 293

ML 281

B5750-25 25 mg
EUR 615

Kvantifikace mRNA pomocí reverzní transkripční PCR v reálném čase (RT-PCR): stylish a problémy.

 The fluorescence-based real-time reverse transcription PCR (RT-PCR) is broadly used for the quantification of steady-state mRNA ranges and is a vital instrument for primary analysis, molecular drugs and biotechnology. Assays are simple to carry out, able to excessive throughput, and may mix excessive sensitivity with dependable specificity. The know-how is evolving quickly with the introduction of latest enzymes, chemistries and instrumentation.

Nonetheless, whereas real-time RT-PCR addresses many of the difficulties inherent in typical RT-PCR, it has develop into more and more clear that it engenders new issues that require pressing consideration. Due to this fact, along with offering a snapshot of the state-of-the-art in real-time RT-PCR, this evaluation has an extra intention: it’ll describe and talk about critically a number of the issues related to deciphering outcomes which can be numerical and lend themselves to statistical evaluation, but whose accuracy is considerably affected by reagent and operator variability.

Bacillus thuringiensis a jeho pesticidní krystalické proteiny.

 Throughout the previous decade the pesticidal bacterium Bacillus thuringiensis has been the topic of intensive analysis. These efforts have yielded appreciable information in regards to the advanced relationships between the construction, mechanism of motion, and genetics of the organism’s pesticidal crystal proteins, and a coherent image of those relationships is starting to emerge.

Different research have centered on the ecological function of the B. thuringiensis crystal proteins, their efficiency in agricultural and different pure settings, and the evolution of resistance mechanisms in goal pests. Armed with this data base and with the instruments of contemporary biotechnology, researchers at the moment are reporting promising leads to engineering more-useful toxins and formulations, in creating transgenic vegetation that categorical pesticidal exercise, and in setting up built-in administration methods to insure that these merchandise are utilized with most effectivity and profit.

NCBI GEO: těžba desítek milionů profilů výrazů – aktualizace databáze a nástrojů.

 The Gene Expression Omnibus (GEO) repository on the Nationwide Heart for Biotechnology Info (NCBI) archives and freely disseminates microarray and different types of high-throughput information generated by the scientific neighborhood. The database has a minimal details about a microarray experiment (MIAME)-compliant infrastructure that captures absolutely annotated uncooked and processed information. A number of information deposit choices and codecs are supported, together with internet varieties, spreadsheets, XML and Easy Omnibus Format in Textual content (SOFT).

Along with information storage, a group of user-friendly web-based interfaces and functions can be found to assist customers successfully discover, visualize and obtain the 1000’s of experiments and tens of hundreds of thousands of gene expression patterns saved in GEO. This paper gives a abstract of the GEO database construction and person services, and describes latest enhancements to database design, efficiency, submission format choices, information question and retrieval utilities. GEO is accessible at http://www.ncbi.nlm.nih.gov/geo/

Glide: nový přístup professional rychlé a přesné dokování a skórování. 2. Faktory obohacení při screeningu databáze.

  • Glide’s capability to determine lively compounds in a database display is characterised by making use of Glide to a various set of 9 protein receptors. In lots of instances, two, and even three, protein websites are employed to probe the sensitivity of the outcomes to the location geometry.
  • To make the database screens as lifelike as doable, the screens use units of “druglike” decoy ligands which have been chosen to be consultant of what we consider is prone to be discovered within the compound assortment of a pharmaceutical or biotechnology firm. Outcomes are introduced for releases 1.8, 2.0, and a couple of.5 of Glide. The comparisons present that common measures for each “early” and “world” enrichment for Glide 2.5 are three instances increased than for Glide 1.Eight and greater than 2 instances increased than for Glide 2.Zero due to higher outcomes for the least well-handled screens.
  • This enchancment in enrichment stems largely from the higher steadiness of the extra broadly parametrized GlideScore 2.5 perform and the inclusion of phrases that penalize ligand-protein interactions that violate established ideas of bodily chemistry, significantly because it issues the publicity to solvent of charged protein and ligand teams.
  • Comparisons to outcomes for the thymidine kinase and estrogen receptors printed by Rognan and associates (J. Med. Chem. 2000, 43, 4759-4767) present that Glide 2.5 performs higher than GOLD 1.1, FlexX 1.8, or DOCK 4.01.

Jednoduchá šifra řídí rozpoznávání DNA pomocí TAL efektorů.

 TAL effectors of plant pathogenic micro organism within the genus Xanthomonas bind host DNA and activate genes that contribute to illness or activate protection. Goal specificity will depend on an effector-variable variety of usually 34 amino acid repeats, however the mechanism of recognition shouldn’t be understood.

We present {that a} repeat-variable pair of residues specifies the nucleotides within the goal web site, one pair to 1 nucleotide, with no obvious context dependence. Our discovering represents a beforehand unknown mechanism for protein-DNA recognition that explains TAL effector specificity, permits goal web site prediction, and opens prospects to be used of TAL effectors in analysis and biotechnology.

Referenční sekvence NCBI (RefSeq): aktuální stav, nové funkce a zásady anotací genomu.

 The Nationwide Heart for Biotechnology Info (NCBI) Reference Sequence (RefSeq) database is a group of genomic, transcript and protein sequence information. These information are chosen and curated from public sequence archives and characterize a major discount in redundancy in comparison with the quantity of information archived by the Worldwide Nucleotide Sequence Database Collaboration. The database consists of over 16,00 organisms, 2.4 × 0(6) genomic information, 13 × 10(6) proteins and a couple of × 10(6) RNA information spanning prokaryotes, eukaryotes and viruses (RefSeq launch 49, September 2011).

The RefSeq database is maintained by a mixed strategy of automated analyses, collaboration and handbook curation to generate an up-to-date illustration of the sequence, its options, names and cross-links to associated sources of data. We report right here on latest development, the standing of curating the human RefSeq information set, extra in depth characteristic annotation and present coverage for eukaryotic genome annotation by way of the NCBI annotation pipeline.

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