• May 10, 2021
alidea

NCBI Reference Sequence (RefSeq):

NCBI Reference Sequence (RefSeq): upravená neredundantní databáze sekvencí genomů, transkriptů a proteinů.

 The Nationwide Heart for Biotechnology Info (NCBI) Reference Sequence (RefSeq) database (http://www.ncbi.nlm.nih.gov/RefSeq/) gives a non-redundant assortment of sequences representing genomic information, transcripts and proteins. Though the objective is to supply a complete dataset representing the whole sequence data for any given species, the database pragmatically consists of sequence information which can be presently publicly accessible within the archival databases. The database incorporates information from over 2400 organisms and consists of over a million proteins representing vital taxonomic variety spanning prokaryotes, eukaryotes and viruses.

Nucleotide and protein sequences are explicitly linked, and the sequences are linked to different assets together with the NCBI Map Viewer and Gene. Sequences are annotated to incorporate coding areas, conserved domains, variation, references, names, database cross-references, and different options utilizing a mixed strategy of collaboration and different enter from the scientific neighborhood, automated annotation, propagation from GenBank and curation by NCBI employees.

alidea
alidea
DAPI, 10mg/mL in H2O
40043 1ML
EUR 134
Description: Minimum order quantity: 1 unit of 1ML
DAPI
B1098-25
EUR 414
DAPI
B1098-5
EUR 142
DAPI
abx082063-10mg 10 mg
EUR 230
  • Shipped within 5-10 working days.
DAPI
40011 10MG
EUR 140
Description: Minimum order quantity: 1 unit of 10MG
50 ML AMPHOTERICIN B, LIQUID 250 µG/ML SOLUBILIZED
30-003-CF 50 mL/pk
EUR 81
Description: Media Catalog; Cell Culture Reagents
50 ML KANAMYCIN SULFATE, LIQUID, 5,000 µG/ML SOLUTION
30-006-CF 50 mL/pk
EUR 98
Description: Media Catalog; Cell Culture Reagents
Amphotericin B (Fungizone) Sol, 250 µg/mL
CCM1120-100 100 mL
EUR 110.23
  • Product category: Culture Media
100 ML ANTIBIOTIC-ANTIMYCOTIC SOLUTION, 10,000 I.U. PENICILLIN (PER ML) 10,000 µG/ML STREPTOMYCIN 25 µG/ML AMPHOTERICIN WITH 8.5 G/L NACI
30-004-CI 100 mL/pk
EUR 103
Description: Media Catalog; Cell Culture Reagents
DAPI (hydrochloride)
C3362-10 10 mg
EUR 122
DAPI (hydrochloride)
C3362-25 25 mg
EUR 200
DAPI (hydrochloride)
C3362-5 5 mg
EUR 93
DAPI (dihydrochloride)
HY-D0814 50mg
EUR 366
DAPI Dilactate
17509 25 mg
EUR 132
  • R-phrase: R20, R21, R68
  • H-Phrase: H303, H313, H340
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 41116134
Latex-in-a-Box Conjugation Kit, Blue 0.3 micron, 7 mL
LIB03-B007 7 mL
EUR 733.94
  • Product Category: Latex Conjugation
Description: Latex-in-a-Box Conjugation Kit conjugates blue 0.3 micron latex nanoparticles to antibodies and proteins. The nanoparticles are created using a special technology that needs only one hour to conjugate the particles to the antibody/protein.
Latex-in-a-Box Conjugation Kit, Red 0.3 micron, 7 mL
LIB03-R007 7 mL
EUR 733.94
  • Product Category: Latex Conjugation
Description: Latex-in-a-Box Conjugation Kit conjugates red 0.3 micron latex nanoparticles to antibodies and proteins. The nanoparticles are created using a special technology that needs only one hour to conjugate the particles to the antibody/protein.
Fluorguard Antifade Mounting Medium
F001 1 ml
EUR 86
Penicillin-Streptomycin 100X Solution, 10, 000 Units/mL, 10, 000 µg/mL
CCM1133-100 100 mL
EUR 64.05
  • Product category: Culture Media
100 ML PENICILLIN-STREPTOMYCIN SOLUTION, 50X, 5,000 I.U. PENICILLIN (PER ML) 5,000 µG/ML STREPTOMYCIN
30-001-CI 100 mL/pk
EUR 82
Description: Media Catalog; Cell Culture Reagents
DAPI Staining Kit
abx097206-1Kit 1 Kit
EUR 230
  • Shipped within 5-10 working days.
FluorLast? with DAPI
1214-20
EUR 153
100 ML PENICILLIN-STREPTOMYCIN-L-GLUTAMINE, 100X, 10,000 I.U. PENCILLIN (PER ML) 10,000 µG/ML STREPTOMYCIN 29.2 MG/ML L-GLUTAMINE
30-009-CI 100 mL/pk
EUR 80
Description: Media Catalog; Cell Culture Reagents
DAPI (Molecular Biology Grade)
CE117 5 mg
EUR 60
DAPI (Molecular Biology Grade)
CE118 25 mg
EUR 133
DAPI (Molecular Biology Grade)
CE119 100 mg
EUR 319
Human Paraoxonase 1 (PON) Standards QQ and RR Phenotypes (2 x 0.3 mL)
0801384 2 x 0.3 mL
EUR 330.8
  • What is the product classification?
  • Human Paraoxonase 1 PON) Standards QQ and RR Phenotypes is marked as RUO.
Description: Please contact Gentaur in order to receive the datasheet of the product.
FluorLast? with DAPI and DABCO
1218-20
EUR 153
EverBrite Mounting Medium with DAPI
23002 10mL
EUR 195
Description: Minimum order quantity: 1 unit of 10mL
0.3 ml 8-Strip Tubes & Domed Caps, 250 Strips of 8 Tubes and Caps
GP209 2,000 tubes/caps
EUR 334
Brilliant Cresyl Blue (0.3%, Alcoholic)
BCA3800 1 Gal.
EUR 241
Brilliant Cresyl Blue (0.3%, Alcoholic)
BCA500 500 ml
EUR 90
Brilliant Cresyl Blue (0.3%, Alcoholic)
BCA999 1000 ml
EUR 116
Anti-Fade Mounting Medium (With DAPI)
abx090690-10ml 10 ml
EUR 203
  • Shipped within 5-10 working days.
EverBrite Hardset Mounting Medium with DAPI
23004 10mL
EUR 213
Description: Minimum order quantity: 1 unit of 10mL
FluoroQuest™ Mounting Medium with DAPI
20004 50 mL
EUR 132
  • R-phrase: R20, R21, R68
  • H-Phrase: H303, H313, H340
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 12352200
Random Hexamers 100 µg
26-4000-03 100 ug
EUR 125
Description:

Random Primers are a mixture of oligonucleotides representing all possible sequence for that size. Random Primers can be used to prime synthesis in oligo-labeling similar to using hexamers (1,2) and cDNA synthesis. Random prime labeling yields high specific activity labeled DNA probe which can be used for all southern, northern and in situ hybridization studies. Random Primers can be also used similar to using hexamers in cDNA synthesis in combination with oligo d(T) to yield more 5' end cDNA sequence. Recently random primers have been used to detect DNA polymorphism. These polymorphisms, simply detected as DNA segments which amplify from one parent but not the other, are inherited in a Mendelian fashion and can be used to construct genetic maps in a variety of species. The authors suggested that these polymorphisms be called RAPD (pronounced RAPID) makers, after Random Amplified Polymorphic DNA (3).

References 1. Feinberg, A.P. & Vogelstein, B. (1983) Anal. Biochem. 132:6-13. 2. Feinberg, A.P. & Vogelstein, B. (1984) Anal. Biochem. 137:266-267. 3. Williams J. G., Kubelik A.R., Livak K.J., Rafalski J.A. & Tingey S.V. (1990) Nucleic Acid Res. 18(22):6531-5.

BrightGreen miRNA qPCR MasterMix-Low ROX
MasterMix-mL 4 x 1.25 ml for 500 reactions
EUR 151
Rat Monoclonal Anti-Mouse CD4 Pur. Low Endotoxin (Clone YTS 191.1) (rat IgG2b)
MCD004-ML 100 ug
EUR 408
Rat Monoclonal Anti-Mouse CD25 , Pur. Low Endotoxin (Clone PC61.5.3) (rat IgG1)
MCD025-ML 100 ug
EUR 408
DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride)
B551 0.2 ml
EUR 108
DiagPoly Amine Polystyrene Particles, 0.3 µm
DNM-F002 10 mL
EUR 580
DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *10 mM solution in water*
17507 2 mL
EUR 115
  • R-phrase: R20, R21, R68
  • H-Phrase: H303, H313, H340
  • Symbol for dangerous compounds: T
  • UNSPEC Code: 41116134
DiagPoly Plain Polystyrene Particles, 0.2-0.3 µm
DNM-P003 10 mL
EUR 554
bFGF-nAb Purified Protein 25 µg
ABP-NAB-BFGFAB 25 µg Ask for price
    • Product line: nAb
    • Brand: Nano Antibodies
bFGF-nAb Plus Biotinylated 25 µg
ABP-NAB-BFGFPB 25 µg Ask for price
    • Product line: nAb
    • Brand: Nano Antibodies
GFP-nAb Purified Protein 25 µg
ABP-NAB-GFPAB 25 µg Ask for price
    • Product line: nAb
    • Brand: Nano Antibodies
GFP-nAb Plus Biotinylated 25 µg
ABP-NAB-GFPPB 25 µg Ask for price
    • Product line: nAb
    • Brand: Nano Antibodies
mNeonGreen-nAb Purified Protein 25 µg
ABP-NAB-MNGAB 25 µg Ask for price
    • Product line: nAb
    • Brand: Nano Antibodies
mNeonGreen-nAb Plus Biotinylated 25 µg
ABP-NAB-MNGPB 25 µg Ask for price
    • Product line: nAb
    • Brand: Nano Antibodies
RFP-nAb Purified Protein 25 µg
ABP-NAB-RFPAB 25 ug Ask for price
    • Product line: nAb
    • Brand: Nano Antibodies
RFP-nAb Plus Biotinylated 25 µg
ABP-NAB-RFPPB 25 µg Ask for price
    • Product line: nAb
    • Brand: Nano Antibodies
Anti-PON1 Clone KRJ1 (100 µg)
0801012 100 µg
EUR 284
  • What is the product classification?
  • Anti-PON1 Clone KRJ1 is marked as RUO.
Description: Please contact Gentaur in order to receive the datasheet of the product.
Anti-PON1 Clone KRJ2 (100 µg)
0801014 100 µg
EUR 284
  • What is the product classification?
  • Anti-PON1 Clone KRJ2 is marked as RUO.
Description: Please contact Gentaur in order to receive the datasheet of the product.
DAPI, dilactate (4’, 6-diamidino-2-phenylindole, dilactate)
40009 10MG
EUR 146
Description: Minimum order quantity: 1 unit of 10MG
DiagPoly Streptavidin Coated Polystyrene Particles, 0.3-0.4 µm
DNM-C005 10 mL
EUR 676
Papain Solution In PBS, 1mg/ml
CA093-002 20ml Ask for price
Ethidium bromide, 10mg/ml in water
GT9211-10ML 10 ml
EUR 70
Acridine Orange, 10mg/mL in water
40039 10ML
EUR 101
Description: Minimum order quantity: 1 unit of 10ML
Hoechst 33258, 10mg/mL in H2O
40044 10ML
EUR 217
Description: Minimum order quantity: 1 unit of 10ML
Hoechst 33342, 10mg/mL in H2O
40046 10ML
EUR 232
Description: Minimum order quantity: 1 unit of 10ML
ML-211
C3647-1 1 mg
EUR 112
Description: IC50: LYPLA1 (17 nM) and the related LYPLA2 (30 nM)ML-211 is a dual inhibitor of LYPLA1 and the related LYPLA2.Lysophospholipase 1 (LYPLA1), a protein palmitoyl thioesterase, is responsible for depalmitoylation of the oncogene HRas.
ML-211
C3647-10 10 mg
EUR 270
Description: IC50: LYPLA1 (17 nM) and the related LYPLA2 (30 nM)ML-211 is a dual inhibitor of LYPLA1 and the related LYPLA2.Lysophospholipase 1 (LYPLA1), a protein palmitoyl thioesterase, is responsible for depalmitoylation of the oncogene HRas.
ML-211
C3647-25 25 mg
EUR 519
Description: IC50: LYPLA1 (17 nM) and the related LYPLA2 (30 nM)ML-211 is a dual inhibitor of LYPLA1 and the related LYPLA2.Lysophospholipase 1 (LYPLA1), a protein palmitoyl thioesterase, is responsible for depalmitoylation of the oncogene HRas.
ML-211
C3647-5 5 mg
EUR 174
Description: IC50: LYPLA1 (17 nM) and the related LYPLA2 (30 nM)ML-211 is a dual inhibitor of LYPLA1 and the related LYPLA2.Lysophospholipase 1 (LYPLA1), a protein palmitoyl thioesterase, is responsible for depalmitoylation of the oncogene HRas.
ML-265
C4181-10 10 mg
EUR 263
Description: ML-265 (TEPP-46) is a potent and selective PKM2 activator [1][2]. Pyruvate kinase catalyzes the final step in glycolysis and transfers the phosphate group from phosphoenolpyruvate (PEP) to adenosine diphosphate (ADP) to yield adenosine triphosphate (ATP) and pyruvate.
ML-265
C4181-25 25 mg
EUR 463
Description: ML-265 (TEPP-46) is a potent and selective PKM2 activator [1][2]. Pyruvate kinase catalyzes the final step in glycolysis and transfers the phosphate group from phosphoenolpyruvate (PEP) to adenosine diphosphate (ADP) to yield adenosine triphosphate (ATP) and pyruvate.
ML-265
C4181-5 5 mg
EUR 180
Description: ML-265 (TEPP-46) is a potent and selective PKM2 activator [1][2]. Pyruvate kinase catalyzes the final step in glycolysis and transfers the phosphate group from phosphoenolpyruvate (PEP) to adenosine diphosphate (ADP) to yield adenosine triphosphate (ATP) and pyruvate.
ML-178
C4287-10 10 mg
EUR 224
Description: ML-178 is a novel and selective S1P4 activator with EC50 value of 46.3 nM [1]. ML-178 is a novel and selective S1P4 activator.
ML-178
C4287-25 25 mg
EUR 441
Description: ML-178 is a novel and selective S1P4 activator with EC50 value of 46.3 nM [1]. ML-178 is a novel and selective S1P4 activator.
ML-178
C4287-5 5 mg
EUR 145
Description: ML-178 is a novel and selective S1P4 activator with EC50 value of 46.3 nM [1]. ML-178 is a novel and selective S1P4 activator.
ML-193
C4531-10 10 mg
EUR 241
Description: IC50: 221 nMML-193 is a GPR55 antagonist. GPR55 is a class A G protein-coupled receptor (GPCR) that has been implicated in inflammatory pain, neuropathic pain, metabolic disorder, bone development, and cancer.
ML-193
C4531-25 25 mg
EUR 485
Description: IC50: 221 nMML-193 is a GPR55 antagonist. GPR55 is a class A G protein-coupled receptor (GPCR) that has been implicated in inflammatory pain, neuropathic pain, metabolic disorder, bone development, and cancer.
ML-193
C4531-5 5 mg
EUR 180
Description: IC50: 221 nMML-193 is a GPR55 antagonist. GPR55 is a class A G protein-coupled receptor (GPCR) that has been implicated in inflammatory pain, neuropathic pain, metabolic disorder, bone development, and cancer.
ML-090
C4746-25 25 mg
EUR 128
ML-090
C4746-50 50 mg
EUR 180
ML-099
C4771-10 10 mg
EUR 270
ML-099
C4771-25 25 mg
EUR 476
ML-099
C4771-5 5 mg
EUR 183
ML-191
C4786-10 10 mg
EUR 270
ML-191
C4786-25 25 mg
EUR 476
ML-191
C4786-5 5 mg
EUR 183
ML-233
C4788-10 10 mg
EUR 270
ML-233
C4788-25 25 mg
EUR 476
ML-233
C4788-5 5 mg
EUR 183
ML-335
C4792-10 10 mg
EUR 231
ML-335
C4792-100 100 mg
EUR 1298
ML-335
C4792-25 25 mg
EUR 448
ML-335
C4792-5 5 mg
EUR 158
ML-335
C4792-5.1 10 mM (in 1mL DMSO)
EUR 168
ML-335
C4792-50 50 mg
EUR 756
ML-030
C4805-10 10 mg
EUR 270
ML-030
C4805-25 25 mg
EUR 476
ML-030
C4805-5 5 mg
EUR 183
ML-098
C4807-10 10 mg
EUR 270
ML-098
C4807-25 25 mg
EUR 476
ML-098
C4807-5 5 mg
EUR 183
ML-243
C4813-10 10 mg
EUR 270
ML-243
C4813-25 25 mg
EUR 476
ML-243
C4813-5 5 mg
EUR 183
ML-097
C4821-10 10 mg
EUR 270
Description: ML-097 (CID-2160985) is a pan activator of Ras-related GTPases [1]. The Ras superfamily of GTPases, which includes Arf, Rho, Ras and Rab GTPase subfamilies, regulate many cellular processes ranging from membrane trafficking to the control of cell proliferation.
ML-097
C4821-25 25 mg
EUR 476
Description: ML-097 (CID-2160985) is a pan activator of Ras-related GTPases [1]. The Ras superfamily of GTPases, which includes Arf, Rho, Ras and Rab GTPase subfamilies, regulate many cellular processes ranging from membrane trafficking to the control of cell proliferation.
ML-097
C4821-5 5 mg
EUR 183
Description: ML-097 (CID-2160985) is a pan activator of Ras-related GTPases [1]. The Ras superfamily of GTPases, which includes Arf, Rho, Ras and Rab GTPase subfamilies, regulate many cellular processes ranging from membrane trafficking to the control of cell proliferation.
ML-297
C5045-10 10 mg
EUR 284
Description: EC50: 0.16 and 1.8 ?M for GIRK1/2 and GIRK1/4, respectively.
ML-297
C5045-50 50 mg
EUR 1038
Description: EC50: 0.16 and 1.8 ?M for GIRK1/2 and GIRK1/4, respectively.
ML-291
C5358-10 10 mg
EUR 219
Description: ML-291 is an apoptosis inducer. Apoptosis, a process of programmed cell death, occurs in multi-cellular organisms. Biochemical events result in characteristic cell changes (morphology) and death.
ML-291
C5358-25 25 mg
EUR 457
Description: ML-291 is an apoptosis inducer. Apoptosis, a process of programmed cell death, occurs in multi-cellular organisms. Biochemical events result in characteristic cell changes (morphology) and death.
ML-291
C5358-5 5 mg
EUR 168
Description: ML-291 is an apoptosis inducer. Apoptosis, a process of programmed cell death, occurs in multi-cellular organisms. Biochemical events result in characteristic cell changes (morphology) and death.
ML-031
C5718-10 10 mg
EUR 270
Description: ML-031 is an agonist of S1P2 with EC50 value of 1 ?M [1].
ML-031
C5718-25 25 mg
EUR 476
Description: ML-031 is an agonist of S1P2 with EC50 value of 1 ?M [1].
ML-031
C5718-5 5 mg
EUR 183
Description: ML-031 is an agonist of S1P2 with EC50 value of 1 ?M [1].
ML-365
B2076-25
EUR 729
ML-365
B2076-5
EUR 218
ML-35
B2077-25
EUR 729
ML-35
B2077-5
EUR 218
ML-162
B2304-1
EUR 142
ML 171
B5672-5.1 10 mM (in 1mL DMSO)
EUR 108
ML 171
B5672-50 50 mg
EUR 125
ML 204
B5696-10 10 mg
EUR 235
ML 204
B5696-25 25 mg
EUR 461
ML 204
B5696-5 5 mg
EUR 152
ML 204
B5696-50 50 mg
EUR 822
ML SA1
B5700-10 10 mg
EUR 226
ML SA1
B5700-50 50 mg
EUR 795
ML 221
B5702-10 10 mg
EUR 210
ML 221
B5702-100 100 mg
EUR 830
ML 221
B5702-25 25 mg
EUR 392
ML 202
B5739-10 10 mg
EUR 299
ML 190
B5741-10 10 mg
EUR 383
ML 190
B5741-50 50 mg
EUR 1455
ML 281
B5750-10 10 mg
EUR 293
ML 281
B5750-25 25 mg
EUR 615
ML 281
B5750-5 5 mg
EUR 206
ML 289
B5783-10 10 mg
EUR 232
ML 289
B5783-25 25 mg
EUR 453
ML 289
B5783-5 5 mg
EUR 151
ML 337
B5786-10 10 mg
EUR 366
ML 337
B5786-50 50 mg
EUR 1347
ML-240
B1142-25
EUR 588
ML-240
B1142-5
EUR 185
ML-323
B1317-25 25 mg
EUR 374
Description: IC50: ML-323 is a highly potent and selective inhibitor of USP1-UAF1 with IC50 value of 76 nM.ML-323 is an inhibitor of USP1-UAF1 with remarkable selectivity and relatively low cytotoxicity to the human cells.
ML-323
B1317-5 5 mg
EUR 125
Description: IC50: ML-323 is a highly potent and selective inhibitor of USP1-UAF1 with IC50 value of 76 nM.ML-323 is an inhibitor of USP1-UAF1 with remarkable selectivity and relatively low cytotoxicity to the human cells.
ML-323
B1317-5.1 10 mM (in 1mL DMSO)
EUR 142
Description: IC50: ML-323 is a highly potent and selective inhibitor of USP1-UAF1 with IC50 value of 76 nM.ML-323 is an inhibitor of USP1-UAF1 with remarkable selectivity and relatively low cytotoxicity to the human cells.
ML 240
B4926-10 10 mg
EUR 270
ML 240
B4926-25 25 mg
EUR 537
ML 240
B4926-5 5 mg
EUR 171
ML 348
B4933-10 10 mg
EUR 279
ML 348
B4933-5.1 10 mM (in 1mL DMSO)
EUR 247
ML 348
B4933-50 50 mg
EUR 1014
ML 349
B4934-10 10 mg
EUR 311
ML 349
B4934-5 5 mg
EUR 206
ML 213
B5628-10 10 mg
EUR 196
ML 213
B5628-25 25 mg
EUR 376
ML 213
B5628-5 5 mg
EUR 126
ML 213
B5628-50 50 mg
EUR 661
ML 145
B7604-10 10 mg
EUR 383
ML 145
B7604-50 50 mg
EUR 1439
ML 277
B7711-10 10 mg
EUR 270
ML 277
B7711-25 25 mg
EUR 476
ML 277
B7711-5 5 mg
EUR 183
ML 154
B7765-10 10 mg
EUR 447

Kvantifikace mRNA pomocí reverzní transkripční PCR v reálném čase (RT-PCR): stylish a problémy.

 The fluorescence-based real-time reverse transcription PCR (RT-PCR) is broadly used for the quantification of steady-state mRNA ranges and is a vital instrument for primary analysis, molecular drugs and biotechnology. Assays are simple to carry out, able to excessive throughput, and may mix excessive sensitivity with dependable specificity. The know-how is evolving quickly with the introduction of latest enzymes, chemistries and instrumentation.

Nonetheless, whereas real-time RT-PCR addresses many of the difficulties inherent in typical RT-PCR, it has develop into more and more clear that it engenders new issues that require pressing consideration. Due to this fact, along with offering a snapshot of the state-of-the-art in real-time RT-PCR, this evaluation has an extra intention: it’ll describe and talk about critically a number of the issues related to deciphering outcomes which can be numerical and lend themselves to statistical evaluation, but whose accuracy is considerably affected by reagent and operator variability.

Bacillus thuringiensis a jeho pesticidní krystalické proteiny.

 Throughout the previous decade the pesticidal bacterium Bacillus thuringiensis has been the topic of intensive analysis. These efforts have yielded appreciable information in regards to the advanced relationships between the construction, mechanism of motion, and genetics of the organism’s pesticidal crystal proteins, and a coherent image of those relationships is starting to emerge.

Different research have centered on the ecological function of the B. thuringiensis crystal proteins, their efficiency in agricultural and different pure settings, and the evolution of resistance mechanisms in goal pests. Armed with this data base and with the instruments of contemporary biotechnology, researchers at the moment are reporting promising leads to engineering more-useful toxins and formulations, in creating transgenic vegetation that categorical pesticidal exercise, and in setting up built-in administration methods to insure that these merchandise are utilized with most effectivity and profit.

NCBI GEO: těžba desítek milionů profilů výrazů – aktualizace databáze a nástrojů.

 The Gene Expression Omnibus (GEO) repository on the Nationwide Heart for Biotechnology Info (NCBI) archives and freely disseminates microarray and different types of high-throughput information generated by the scientific neighborhood. The database has a minimal details about a microarray experiment (MIAME)-compliant infrastructure that captures absolutely annotated uncooked and processed information. A number of information deposit choices and codecs are supported, together with internet varieties, spreadsheets, XML and Easy Omnibus Format in Textual content (SOFT).

Along with information storage, a group of user-friendly web-based interfaces and functions can be found to assist customers successfully discover, visualize and obtain the 1000’s of experiments and tens of hundreds of thousands of gene expression patterns saved in GEO. This paper gives a abstract of the GEO database construction and person services, and describes latest enhancements to database design, efficiency, submission format choices, information question and retrieval utilities. GEO is accessible at http://www.ncbi.nlm.nih.gov/geo/

Glide: nový přístup professional rychlé a přesné dokování a skórování. 2. Faktory obohacení při screeningu databáze.

  • Glide’s capability to determine lively compounds in a database display is characterised by making use of Glide to a various set of 9 protein receptors. In lots of instances, two, and even three, protein websites are employed to probe the sensitivity of the outcomes to the location geometry.
  • To make the database screens as lifelike as doable, the screens use units of “druglike” decoy ligands which have been chosen to be consultant of what we consider is prone to be discovered within the compound assortment of a pharmaceutical or biotechnology firm. Outcomes are introduced for releases 1.8, 2.0, and a couple of.5 of Glide. The comparisons present that common measures for each “early” and “world” enrichment for Glide 2.5 are three instances increased than for Glide 1.Eight and greater than 2 instances increased than for Glide 2.Zero due to higher outcomes for the least well-handled screens.
  • This enchancment in enrichment stems largely from the higher steadiness of the extra broadly parametrized GlideScore 2.5 perform and the inclusion of phrases that penalize ligand-protein interactions that violate established ideas of bodily chemistry, significantly because it issues the publicity to solvent of charged protein and ligand teams.
  • Comparisons to outcomes for the thymidine kinase and estrogen receptors printed by Rognan and associates (J. Med. Chem. 2000, 43, 4759-4767) present that Glide 2.5 performs higher than GOLD 1.1, FlexX 1.8, or DOCK 4.01.

Jednoduchá šifra řídí rozpoznávání DNA pomocí TAL efektorů.

 TAL effectors of plant pathogenic micro organism within the genus Xanthomonas bind host DNA and activate genes that contribute to illness or activate protection. Goal specificity will depend on an effector-variable variety of usually 34 amino acid repeats, however the mechanism of recognition shouldn’t be understood.

We present {that a} repeat-variable pair of residues specifies the nucleotides within the goal web site, one pair to 1 nucleotide, with no obvious context dependence. Our discovering represents a beforehand unknown mechanism for protein-DNA recognition that explains TAL effector specificity, permits goal web site prediction, and opens prospects to be used of TAL effectors in analysis and biotechnology.

Referenční sekvence NCBI (RefSeq): aktuální stav, nové funkce a zásady anotací genomu.

 The Nationwide Heart for Biotechnology Info (NCBI) Reference Sequence (RefSeq) database is a group of genomic, transcript and protein sequence information. These information are chosen and curated from public sequence archives and characterize a major discount in redundancy in comparison with the quantity of information archived by the Worldwide Nucleotide Sequence Database Collaboration. The database consists of over 16,00 organisms, 2.4 × 0(6) genomic information, 13 × 10(6) proteins and a couple of × 10(6) RNA information spanning prokaryotes, eukaryotes and viruses (RefSeq launch 49, September 2011).

The RefSeq database is maintained by a mixed strategy of automated analyses, collaboration and handbook curation to generate an up-to-date illustration of the sequence, its options, names and cross-links to associated sources of data. We report right here on latest development, the standing of curating the human RefSeq information set, extra in depth characteristic annotation and present coverage for eukaryotic genome annotation by way of the NCBI annotation pipeline.

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