Abirateron u metastazujícího karcinomu prostaty
Abirateron u metastazujícího karcinomu prostaty bez předchozí chemoterapie.
Abiraterone acetate, an androgen biosynthesis inhibitor, improves total survival in sufferers with metastatic castration-resistant prostate most cancers after chemotherapy. We evaluated this agent in sufferers who had not acquired earlier chemotherapy.
METHODS
On this double-blind examine, we randomly assigned 1088 sufferers to obtain abiraterone acetate (1000 mg) plus prednisone (5 mg twice day by day) or placebo plus prednisone. The coprimary finish factors have been radiographic progression-free survival and total survival.
RESULTS
The examine was unblinded after a deliberate interim evaluation that was carried out after 43% of the anticipated deaths had occurred. The median radiographic progression-free survival was 16.5 months with abiraterone-prednisone and eight.three months with prednisone alone (hazard ratio for abiraterone-prednisone vs. prednisone alone, 0.53; 95% confidence interval [CI], 0.45 to 0.62; P<0.001). Over a median follow-up interval of 22.2 months, total survival was improved with abiraterone-prednisone (median not reached, vs. 27.2 months for prednisone alone; hazard ratio, 0.75; 95% CI, 0.61 to 0.93; P=0.01) however didn’t cross the efficacy boundary.
Abiraterone-prednisone confirmed superiority over prednisone alone with respect to time to initiation of cytotoxic chemotherapy, opiate use for cancer-related ache, prostate-specific antigen development, and decline in efficiency standing. Grade three or four mineralocorticoid-related adversarial occasions and abnormalities on liver-function testing have been extra widespread with abiraterone-prednisone.
CONCLUSIONS
Abiraterone improved radiographic progression-free survival, confirmed a pattern towards improved total survival, and considerably delayed scientific decline and initiation of chemotherapy in sufferers with metastatic castration-resistant prostate most cancers. (Funded by Janssen Analysis and Improvement, previously Cougar Biotechnology; ClinicalTrials.gov quantity, NCT00887198.).
 PI-103 |
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| 1728-1 | Biovision | EUR 153 | |
 ELISA Kit) Human Proinsulin (PI) ELISA Kit |
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| RD-PI-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 478 |
Human Proinsulin (PI) ELISA Kit |
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| RD-PI-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 662 |
 ELISA Kit) Porcine Proinsulin (PI) ELISA Kit |
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| RD-PI-p-48Tests | Reddot Biotech | 48 Tests | EUR 555 |
Porcine Proinsulin (PI) ELISA Kit |
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| RD-PI-p-96Tests | Reddot Biotech | 96 Tests | EUR 771 |
Human Proinsulin (PI) ELISA Kit |
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| DLR-PI-Hu-48T | DL Develop | 48T | EUR 479 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human Proinsulin (PI) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Human Proinsulin (PI) ELISA Kit |
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| DLR-PI-Hu-96T | DL Develop | 96T | EUR 621 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human Proinsulin (PI) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Porcine Proinsulin (PI) ELISA Kit |
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| DLR-PI-p-48T | DL Develop | 48T | EUR 547 |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Porcine Proinsulin (PI) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Porcine Proinsulin (PI) ELISA Kit |
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| DLR-PI-p-96T | DL Develop | 96T | EUR 715 |
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Description: A competitive inhibition quantitative ELISA assay kit for detection of Porcine Proinsulin (PI) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Human Proinsulin (PI) ELISA Kit |
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| RDR-PI-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 500 |
Human Proinsulin (PI) ELISA Kit |
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| RDR-PI-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 692 |
Porcine Proinsulin (PI) ELISA Kit |
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| RDR-PI-p-48Tests | Reddot Biotech | 48 Tests | EUR 580 |
Porcine Proinsulin (PI) ELISA Kit |
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| RDR-PI-p-96Tests | Reddot Biotech | 96 Tests | EUR 807 |
 10,000 µG/ML STREPTOMYCIN 25 µG/ML AMPHOTERICIN WITH 8.5 G/L NACI) 100 ML ANTIBIOTIC-ANTIMYCOTIC SOLUTION, 10,000 I.U. PENICILLIN (PER ML) 10,000 µG/ML STREPTOMYCIN 25 µG/ML AMPHOTERICIN WITH 8.5 G/L NACI |
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| 30-004-CI | CORNING | 100 mL/pk | EUR 103 |
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Description: Media Catalog; Cell Culture Reagents |
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) dNTP Mix (1 ml) |
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| 9002-1 | Biovision | EUR 153 | |
 Fluorguard Antifade Mounting Medium |
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| F001 | Insitus Biotechnologies | 1 ml | EUR 86 |
 Penicillin-Streptomycin 100X Solution, 10, 000 Units/mL, 10, 000 µg/mL |
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| CCM1133-100 | Bio Basic | 100 mL | EUR 64.05 |
 5,000 µG/ML STREPTOMYCIN) 100 ML PENICILLIN-STREPTOMYCIN SOLUTION, 50X, 5,000 I.U. PENICILLIN (PER ML) 5,000 µG/ML STREPTOMYCIN |
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| 30-001-CI | CORNING | 100 mL/pk | EUR 82 |
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Description: Media Catalog; Cell Culture Reagents |
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 ML-211 |
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| C3647-1 | ApexBio | 1 mg | EUR 112 |
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Description: IC50: LYPLA1 (17 nM) and the related LYPLA2 (30 nM)ML-211 is a dual inhibitor of LYPLA1 and the related LYPLA2.Lysophospholipase 1 (LYPLA1), a protein palmitoyl thioesterase, is responsible for depalmitoylation of the oncogene HRas. |
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 ML-356 |
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| C3354-1 | ApexBio | 1 mg | EUR 126 |
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Description: ML-356 is an inhibitor of the thioesterase domain of fatty acid synthase with IC50 value of 334 nM.Fatty acid synthase (FAS) is a multi-enzyme protein that catalyzes fatty acid synthesis, fatty acids are related to energy production and storage, cellular structure ,hormones biosynthesis |
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 ML-162 |
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| B2304-1 | Biovision | EUR 142 | |
 ML-277 |
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| B2730-1 | Biovision | 1 mg | EUR 115 |
 ML-324 |
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| 2763-1 | Biovision | EUR 131 | |
 (1 ml)) HIV Infection Enhancer (Medium) (1 ml) |
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| H901-1 | 101Bio | - | Ask for price |
 10,000 µG/ML STREPTOMYCIN 29.2 MG/ML L-GLUTAMINE) 100 ML PENICILLIN-STREPTOMYCIN-L-GLUTAMINE, 100X, 10,000 I.U. PENCILLIN (PER ML) 10,000 µG/ML STREPTOMYCIN 29.2 MG/ML L-GLUTAMINE |
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| 30-009-CI | CORNING | 100 mL/pk | EUR 80 |
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Description: Media Catalog; Cell Culture Reagents |
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 Anti-GST3/GST pi/Gstp1 Antibody |
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| A00394-1 | BosterBio | 100ug/vial | EUR 334 |
, Sterile-Filtered) StemBoost? SMAD Signaling Inhibitor Cocktail Set (1000X), Sterile-Filtered |
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| K877-1-ML | Biovision | EUR 153 | |
) Kanamycin solution (50mg/ml) |
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| MN800A-1 | SBI | 20 ml | EUR 127 |
) Camptothecin (2 mg/ml) |
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| 1039-1 | Biovision | EUR 142 | |
) NATtrol Respiratory Pathogen Panel -1 (6 x 0.25 mL) |
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| NATRPP-1 | Zeptometrix | 6 x 0.25 mL | EUR 464.96 |
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Description: Please contact Gentaur in order to receive the datasheet of the product. |
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, 2mg/mL) CF350 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
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| 20245-1 | Biotium | 50uL | EUR 149 |
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Description: Minimum order quantity: 1 unit of 50uL |
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, 2mg/mL) CF488A Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
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| 20246-1 | Biotium | 50uL | EUR 149 |
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Description: Minimum order quantity: 1 unit of 50uL |
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, 2mg/mL) CF555 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
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| 20247-1 | Biotium | 50uL | EUR 149 |
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Description: Minimum order quantity: 1 unit of 50uL |
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, 2mg/mL) CF568 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
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| 20248-1 | Biotium | 50uL | EUR 149 |
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Description: Minimum order quantity: 1 unit of 50uL |
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, 2mg/mL) CF594 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
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| 20249-1 | Biotium | 50uL | EUR 149 |
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Description: Minimum order quantity: 1 unit of 50uL |
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, 2mg/mL) CF633 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
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| 20250-1 | Biotium | 50uL | EUR 149 |
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Description: Minimum order quantity: 1 unit of 50uL |
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, 2mg/mL) CF640R Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
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| 20251-1 | Biotium | 50uL | EUR 149 |
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Description: Minimum order quantity: 1 unit of 50uL |
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, 2mg/mL) CF647 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
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| 20252-1 | Biotium | 50uL | EUR 149 |
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Description: Minimum order quantity: 1 unit of 50uL |
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, 2mg/mL) CF680 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
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| 20253-1 | Biotium | 50uL | EUR 161 |
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Description: Minimum order quantity: 1 unit of 50uL |
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, 2mg/mL) CF770 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
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| 20254-1 | Biotium | 50uL | EUR 161 |
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Description: Minimum order quantity: 1 unit of 50uL |
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, 2mg/mL) CF543 Goat Anti-Mouse IgG1 (γ1), 2mg/mL |
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| 20325-1 | Biotium | 50uL | EUR 149 |
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Description: Minimum order quantity: 1 unit of 50uL |
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 PI |
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| abx290031-400tests | Abbexa | 400 tests | EUR 258 |
 pI |
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| EF007077 | Lifescience Market | 96 Tests | EUR 689 |
 Rabbit Brain Cephalin 5 mL |
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| 41052-1 | Pel-Freez | 5mL | EUR 186.8 |
) AXYPREP MAG DYECLEAN KIT- 1 ML - (SAMPLE SIZE) |
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| MAG-DYECL-1 | CORNING | 1/pk | EUR 75 |
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Description: Bioscience Mag Beads; Magnetic Dye |
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 Arachidonic Acid 5mg/ml |
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| ABP-ARA-1 | Abpcorp | 3 x 0,5ml vials | EUR 57 |
 PI/ Rat PI ELISA Kit |
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| ELA-E0379r | Lifescience Market | 96 Tests | EUR 886 |
) ExoQuick exosome precipitation solution (20 ml) |
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| EXOQ20A-1 | SBI | 20 ml | EUR 1179 |
) ExoQuick exosome precipitation solution (5 ml) |
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| EXOQ5A-1 | SBI | 5 ml | EUR 400 |
) AAVancedTM Concentration Reagent (100 ml aliquot) |
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| AAV100A-1 | SBI | 100 ml | EUR 521 |
) AAVancedTM Concentration Reagent (250 ml aliquot) |
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| AAV110A-1 | SBI | 250 ml | EUR 999 |
 GSTP2 Glutathione S-Transferase pi 2 Mouse Recombinant Protein |
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| PROTP46425-1 | BosterBio | Regular: 20ug | EUR 317 |
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Description: GSTP2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 210 amino acids (1-210 a.a.) and having a molecular mass of 23.5kDa.; The GSTP2 is purified by proprietary chromatographic techniques. |
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 Random Hexamers 100 µg |
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| 26-4000-03 | Gene Link | 100 ug | EUR 125 |
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Description: Random Primers are a mixture of oligonucleotides representing all possible sequence for that size. Random Primers can be used to prime synthesis in oligo-labeling similar to using hexamers (1,2) and cDNA synthesis. Random prime labeling yields high specific activity labeled DNA probe which can be used for all southern, northern and in situ hybridization studies. Random Primers can be also used similar to using hexamers in cDNA synthesis in combination with oligo d(T) to yield more 5' end cDNA sequence. Recently random primers have been used to detect DNA polymorphism. These polymorphisms, simply detected as DNA segments which amplify from one parent but not the other, are inherited in a Mendelian fashion and can be used to construct genetic maps in a variety of species. The authors suggested that these polymorphisms be called RAPD (pronounced RAPID) makers, after Random Amplified Polymorphic DNA (3). References 1. Feinberg, A.P. & Vogelstein, B. (1983) Anal. Biochem. 132:6-13. 2. Feinberg, A.P. & Vogelstein, B. (1984) Anal. Biochem. 137:266-267. 3. Williams J. G., Kubelik A.R., Livak K.J., Rafalski J.A. & Tingey S.V. (1990) Nucleic Acid Res. 18(22):6531-5. |
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 (rat IgG2b)) Rat Monoclonal Anti-Mouse CD4 Pur. Low Endotoxin (Clone YTS 191.1) (rat IgG2b) |
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| MCD004-ML | Alpha Diagnostics | 100 ug | EUR 408 |
 (rat IgG1)) Rat Monoclonal Anti-Mouse CD25 , Pur. Low Endotoxin (Clone PC61.5.3) (rat IgG1) |
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| MCD025-ML | Alpha Diagnostics | 100 ug | EUR 408 |
 BrightGreen miRNA qPCR MasterMix-Low ROX |
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| MasterMix-mL | ABM | 4 x 1.25 ml for 500 reactions | EUR 151 |
) AXYPREP FRAGMENTSELECT-I KIT- 1 ML - (SAMPLE SIZE) |
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| MAG-FRAG-I-1 | CORNING | 1/pk | EUR 64 |
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Description: Bioscience Mag Beads; Magnetic Fragment Select |
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) Rabbit Complement 3-4 WK (10-mL) |
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| 31061-1 | Pel-Freez | 10mL | EUR 279.24 |
 PI-828 |
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| HY-108606 | MedChemExpress | 1mg | EUR 142 |
 PI-3065 |
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| HY-12235 | MedChemExpress | 10mM/1mL | EUR 204 |
 PI-1840 |
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| HY-12286 | MedChemExpress | 50mg | EUR 587 |
PI-103 |
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| HY-10115 | MedChemExpress | 100mg | EUR 546 |
PI-103 |
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| E1KS1038 | EnoGene | 5mg | EUR 325 |
 PI / RNAse |
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| abx290029-200tests | Abbexa | 200 tests | EUR 272 |
PI-3065 |
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| B4660-10 | ApexBio | 10 mg | EUR 224 |
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Description: PI-3065 is a small molecule and selective inhibitor of p110?kinase with an Ki value of 1.5nM and IC50 value of 5nM [1].PI-3065 has been reported to selectively inhibit p110?kinase with Ki values of 15nM, 110nM, 130nM and 940nM |
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PI-3065 |
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| B4660-100 | ApexBio | 100 mg | EUR 792 |
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Description: PI-3065 is a small molecule and selective inhibitor of p110?kinase with an Ki value of 1.5nM and IC50 value of 5nM [1].PI-3065 has been reported to selectively inhibit p110?kinase with Ki values of 15nM, 110nM, 130nM and 940nM |
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PI-3065 |
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| B4660-5 | ApexBio | 5 mg | EUR 166 |
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Description: PI-3065 is a small molecule and selective inhibitor of p110?kinase with an Ki value of 1.5nM and IC50 value of 5nM [1].PI-3065 has been reported to selectively inhibit p110?kinase with Ki values of 15nM, 110nM, 130nM and 940nM |
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PI-3065 |
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| B4660-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 177 |
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Description: PI-3065 is a small molecule and selective inhibitor of p110?kinase with an Ki value of 1.5nM and IC50 value of 5nM [1].PI-3065 has been reported to selectively inhibit p110?kinase with Ki values of 15nM, 110nM, 130nM and 940nM |
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PI-3065 |
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| B4660-50 | ApexBio | 50 mg | EUR 572 |
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Description: PI-3065 is a small molecule and selective inhibitor of p110?kinase with an Ki value of 1.5nM and IC50 value of 5nM [1].PI-3065 has been reported to selectively inhibit p110?kinase with Ki values of 15nM, 110nM, 130nM and 940nM |
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PI-1840 |
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| B4755-10 | ApexBio | 10 mg | EUR 184 |
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Description: PI-1840 is a potent and selective inhibitor of proteasome with IC50 value of 27 nM for chymotrypsinlike (CT-L) activity [1]. The 26S proteasome consists of a 19S regulatory particle (RP) and a 20S core particle. |
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PI-1840 |
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| B4755-50 | ApexBio | 50 mg | EUR 522 |
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Description: PI-1840 is a potent and selective inhibitor of proteasome with IC50 value of 27 nM for chymotrypsinlike (CT-L) activity [1]. The 26S proteasome consists of a 19S regulatory particle (RP) and a 20S core particle. |
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PI-103 |
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| A2067-25 | ApexBio | 25 mg | EUR 328 |
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Description: PI-103 is a potent and selective inhibitor of class I PI3K, mTOR and DNA-PK with IC50 values of 2, 3, 3, 15, 30 and 23 nM for p110?, p110?, p110?, p110?, mTOR and DNA-PK, respectively [1]. |
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PI-103 |
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| A2067-5 | ApexBio | 5 mg | EUR 147 |
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Description: PI-103 is a potent and selective inhibitor of class I PI3K, mTOR and DNA-PK with IC50 values of 2, 3, 3, 15, 30 and 23 nM for p110?, p110?, p110?, p110?, mTOR and DNA-PK, respectively [1]. |
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PI-103 |
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| A2067-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 154 |
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Description: PI-103 is a potent and selective inhibitor of class I PI3K, mTOR and DNA-PK with IC50 values of 2, 3, 3, 15, 30 and 23 nM for p110?, p110?, p110?, p110?, mTOR and DNA-PK, respectively [1]. |
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PI-103 |
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| A2067-S | ApexBio | Evaluation Sample | EUR 81 |
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Description: PI-103 is a potent and selective inhibitor of class I PI3K, mTOR and DNA-PK with IC50 values of 2, 3, 3, 15, 30 and 23 nM for p110?, p110?, p110?, p110?, mTOR and DNA-PK, respectively [1]. |
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 PI 828 |
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| B7198-10 | ApexBio | 10 mg | EUR 318 |
PI 828 |
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| B7198-5 | ApexBio | 5 mg | EUR 203 |
PI-3065 |
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| B1674-25 | Biovision | EUR 506 | |
PI-3065 |
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| B1674-5 | Biovision | EUR 158 | |
PI-103 |
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| 1728-5 | Biovision | EUR 419 | |
PI-103 |
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| 1728-50 | Biovision | Ask for price | |
) ExoQuick Plasma prep and Exosome precipitation kit (5 ml ExoQuick plus 500 ul Thrombin at 500U/mL) |
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| EXOQ5TM-1 | SBI | 75 reactions | EUR 843 |
) Steady-Luc Firefly HTS Assay Kit (12 ml) |
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| 30028-1 | Biotium | 1KIT | EUR 139 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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) PEG-it Virus Precipitation Solution (100 mL aliquot) |
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| LV810A-1 | SBI | 100 ml | EUR 382 |
) PEG-it Virus Precipitation Solution (250 mL aliquot) |
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| LV825A-1 | SBI | 250 ml | EUR 777 |
, 500ul) Water-soluble Cumate solution (300mg/ml, 10,000x), 500ul |
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| QM150A-1 | SBI | 500 ul | EUR 164 |
) AXYPREP MAG PCR NORMALIZER KIT- 1 ML - (SAMPLE SIZE) |
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| MAG-PCR-NM-1 | CORNING | 1/pk | EUR 73 |
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Description: Bioscience Mag Beads; Magnetic PCR Normaliser |
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 Antibody) Glutathione S-Transferase Pi (GST pi) Antibody |
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| abx020559-10ug | Abbexa | 10 ug | EUR 342 |
) Human Anti-HIV-1 Polyclonal Antibody (500 µg) |
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| 0801040 | Zeptometrix | 500 µg | EUR 450.4 |
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Description: Please contact Gentaur in order to receive the datasheet of the product. |
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, 2mg/mL) CF555 goat anti-rabbit IgG(H+L), 2mg/mL |
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| 20033-1 | Biotium | 50uL | EUR 120 |
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Description: Minimum order quantity: 1 unit of 50uL |
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Kvantifikace mRNA pomocí RT-PCR v reálném čase.
The actual-time reverse transcription polymerase chain response (RT-qPCR) addresses the evident requirement for quantitative knowledge evaluation in molecular drugs, biotechnology, microbiology and diagnostics and has develop into the strategy of alternative for the quantification of mRNA. Though it’s typically described as a “gold” commonplace, it’s removed from being a regular assay. The numerous issues brought on by variability of RNA templates, assay designs and protocols, in addition to inappropriate knowledge normalization and inconsistent knowledge evaluation, are broadly recognized but in addition broadly disregarded.
As a primary step in the direction of standardization, we describe a collection of RT-qPCR protocols that illustrate the important technical steps required to generate quantitative knowledge which can be dependable and reproducible. We want to emphasize, nonetheless, that RT-qPCR knowledge represent solely a snapshot of data concerning the amount of a given transcript in a cell or tissue. Any evaluation of the organic penalties of variable mRNA ranges should embrace extra data concerning regulatory RNAs, protein ranges and protein exercise. All the protocol described right here, encompassing all phases from preliminary assay design to dependable qPCR knowledge evaluation, requires roughly 15 h.
NCBI GEO: archiv professional funkční datové soubory genomiky – po 10 letech.
A decade in the past, the Gene Expression Omnibus (GEO) database was established on the Nationwide Heart for Biotechnology Info (NCBI). The unique goal of GEO was to function a public repository for high-throughput gene expression knowledge generated largely by microarray expertise. Nevertheless, the analysis neighborhood shortly utilized microarrays to non-gene-expression research, together with examination of genome copy quantity variation and genome-wide profiling of DNA-binding proteins. As a result of the GEO database was designed with a versatile construction, it was potential to shortly adapt the repository to retailer these knowledge varieties. Extra lately, because the microarray neighborhood switches to next-generation sequencing applied sciences, GEO has once more tailored to host these knowledge units.
At the moment, GEO shops over 20,000 microarray- and sequence-based purposeful genomics research, and continues to deal with nearly all of direct high-throughput knowledge submissions from the analysis neighborhood. A number of mechanisms are supplied to assist customers successfully search, browse, obtain and visualize the information on the degree of particular person genes or whole research. This paper describes latest database enhancements, together with new search and knowledge illustration instruments, in addition to a short assessment of how the neighborhood makes use of GEO knowledge.
Vývoj řady binárních vektorů brány, pGWB, professional realizaci efektivní konstrukce fúzních genů professional transformaci rostlin.
- We developed a brand new collection of binary vectors helpful for Gateway cloning to facilitate transgenic experiments in plant biotechnology. The brand new system, Gateway Binary Vectors (pGWBs) realized environment friendly cloning, constitutive expression utilizing the cauliflower mosaic virus (CaMV) 35S promoter and the development of fusion genes by easy clonase response with an entry clone.
- The reporters employable on this system are beta-glucuronidase (GUS), artificial inexperienced fluorescent protein with S65T mutation (sGFP), luciferase (LUC), enhanced yellow fluorescent protein (EYFP), and enhanced cyan fluorescent protein (ECFP). The tags accessible are 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7-epitope, and tandem affinity purification (TAP). In complete, 13 sorts of reporter or tag have been organized and have been nearly relevant to each N- and C-fusions.
- The pGWBs could possibly be used for a lot of functions, reminiscent of promoter::reporter evaluation, statement of subcellular localization by the expression of proteins fused to a reporter or tag, and evaluation of protein-protein interplay by copurification and immunodetection experiments.
- The pGWBs have been constructed with modified pBI101 containing a CaMV35S promoter-driven hygromycin phosphotransferase (HPT) gene because the second choice marker. We additionally constructed pGWBs with the marker HPT pushed by the nopaline synthase promoter. Through the use of the pGWB system, the expression of tagged proteins, and the localization of GFP-fused proteins have been simply analyzed. Furthermore, tissue-specific and inducible gene expression utilizing a promoter was additionally monitored with pGWBs. It’s anticipated that, the pGWB system will function a robust device for plasmid development in plant analysis.
NCBI GEO: archiv professional vysoce výkonná funkční genomová knowledge.
The Gene Expression Omnibus (GEO) on the Nationwide Heart for Biotechnology Info (NCBI) is the most important public repository for high-throughput gene expression knowledge. Moreover, GEO hosts different classes of high-throughput purposeful genomic knowledge, together with people who study genome copy quantity variations, chromatin construction, methylation standing and transcription issue binding. These knowledge are generated by the analysis neighborhood utilizing high-throughput applied sciences like microarrays and, extra lately, next-generation sequencing.
The database has a versatile infrastructure that may seize absolutely annotated uncooked and processed knowledge, enabling compliance with main community-derived scientific reporting requirements reminiscent of ‘Minimal Info A few Microarray Experiment’ (MIAME). Along with serving as a centralized knowledge storage hub, GEO affords many instruments and options that permit customers to successfully discover, analyze and obtain expression knowledge from each gene-centric and experiment-centric views. This text summarizes the GEO repository construction, content material and working procedures, in addition to lately launched knowledge mining options.
