• 30 května, 2021
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Abirateron u metastazujícího karcinomu prostaty

Abirateron u metastazujícího karcinomu prostaty bez předchozí chemoterapie.

Abiraterone acetate, an androgen biosynthesis inhibitor, improves total survival in sufferers with metastatic castration-resistant prostate most cancers after chemotherapy. We evaluated this agent in sufferers who had not acquired earlier chemotherapy.

METHODS

On this double-blind examine, we randomly assigned 1088 sufferers to obtain abiraterone acetate (1000 mg) plus prednisone (5 mg twice day by day) or placebo plus prednisone. The coprimary finish factors have been radiographic progression-free survival and total survival.

RESULTS

The examine was unblinded after a deliberate interim evaluation that was carried out after 43% of the anticipated deaths had occurred. The median radiographic progression-free survival was 16.5 months with abiraterone-prednisone and eight.three months with prednisone alone (hazard ratio for abiraterone-prednisone vs. prednisone alone, 0.53; 95% confidence interval [CI], 0.45 to 0.62; P<0.001). Over a median follow-up interval of 22.2 months, total survival was improved with abiraterone-prednisone (median not reached, vs. 27.2 months for prednisone alone; hazard ratio, 0.75; 95% CI, 0.61 to 0.93; P=0.01) however didn’t cross the efficacy boundary.

Abiraterone-prednisone confirmed superiority over prednisone alone with respect to time to initiation of cytotoxic chemotherapy, opiate use for cancer-related ache, prostate-specific antigen development, and decline in efficiency standing. Grade three or four mineralocorticoid-related adversarial occasions and abnormalities on liver-function testing have been extra widespread with abiraterone-prednisone.

CONCLUSIONS

Abiraterone improved radiographic progression-free survival, confirmed a pattern towards improved total survival, and considerably delayed scientific decline and initiation of chemotherapy in sufferers with metastatic castration-resistant prostate most cancers. (Funded by Janssen Analysis and Improvement, previously Cougar Biotechnology; ClinicalTrials.gov quantity, NCT00887198.).

 

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Human Proinsulin (PI) ELISA Kit

DLR-PI-Hu-48T 48T
EUR 574.8
Description: A sandwich quantitative ELISA assay kit for detection of Human Proinsulin (PI) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Proinsulin (PI) ELISA Kit

DLR-PI-Hu-96T 96T
EUR 745.2
Description: A sandwich quantitative ELISA assay kit for detection of Human Proinsulin (PI) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Porcine Proinsulin (PI) ELISA Kit

DLR-PI-p-48T 48T
EUR 656.4
Description: A competitive inhibition quantitative ELISA assay kit for detection of Porcine Proinsulin (PI) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Porcine Proinsulin (PI) ELISA Kit

DLR-PI-p-96T 96T
EUR 858
Description: A competitive inhibition quantitative ELISA assay kit for detection of Porcine Proinsulin (PI) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Proinsulin (PI) ELISA Kit

RD-PI-Hu-48Tests 48 Tests
EUR 573.6

Human Proinsulin (PI) ELISA Kit

RD-PI-Hu-96Tests 96 Tests
EUR 794.4

Porcine Proinsulin (PI) ELISA Kit

RD-PI-p-48Tests 48 Tests
EUR 666

Porcine Proinsulin (PI) ELISA Kit

RD-PI-p-96Tests 96 Tests
EUR 925.2

Human Proinsulin (PI) ELISA Kit

RDR-PI-Hu-48Tests 48 Tests
EUR 600

Human Proinsulin (PI) ELISA Kit

RDR-PI-Hu-96Tests 96 Tests
EUR 830.4

Porcine Proinsulin (PI) ELISA Kit

RDR-PI-p-48Tests 48 Tests
EUR 696

Porcine Proinsulin (PI) ELISA Kit

RDR-PI-p-96Tests 96 Tests
EUR 968.4

Amphotericin B (Fungizone) Sol, 250 µg/mL

CCM1120-100 100 mL
EUR 132.28

Fluorguard Antifade Mounting Medium

F001 1 ml
EUR 103.2

dNTP Mix (1 ml)

9002-1
EUR 183.6

Penicillin-Streptomycin 100X Solution, 10, 000 Units/mL, 10, 000 µg/mL

CCM1133-100 100 mL
EUR 76.86

ML-324

2763-1
EUR 157.2

ML-162

B2304-1
EUR 170.4

ML-277

B2730-1 1 mg
EUR 138

ML-356

C3354-1 1 mg
EUR 151.2
Description: ML-356 is an inhibitor of the thioesterase domain of fatty acid synthase with IC50 value of 334 nM.Fatty acid synthase (FAS) is a multi-enzyme protein that catalyzes fatty acid synthesis, fatty acids are related to energy production and storage, cellular structure ,hormones biosynthesis

ML-211

C3647-1 1 mg
EUR 134.4
Description: IC50: LYPLA1 (17 nM) and the related LYPLA2 (30 nM)ML-211 is a dual inhibitor of LYPLA1 and the related LYPLA2.Lysophospholipase 1 (LYPLA1), a protein palmitoyl thioesterase, is responsible for depalmitoylation of the oncogene HRas.

HIV Infection Enhancer (Medium) (1 ml)

H901-1 - Ask for price

Anti-GST3/GST pi/Gstp1 Antibody

A00394-1 100ug/vial
EUR 400.8

Pi-minimal Screen HTS (1.7 ml per well)

M-CS-211L 96 solutions
EUR 508
Description: Pi-minimal Screen HTS (1.7 ml per well)

Pi-PEG Screen HTS (1.7 ml per well)

M-CS-212L 96 solutions
EUR 508
Description: Pi-PEG Screen HTS (1.7 ml per well)

100 ML PENICILLIN-STREPTOMYCIN-L-GLUTAMINE, 100X, 10,000 I.U. PENCILLIN (PER ML) 10,000 µG/ML STREPTOMYCIN 29.2 MG/ML L-GLUTAMINE

30-009-CI 100 mL/pk
EUR 96
Description: Media Catalog; Cell Culture Reagents

Modular In Situ-1 96 Well Crystallization Plate

ML-MIS-FP3D 1 PLATE
EUR 392
Description: Modular In Situ-1 96 Well Crystallization Plate

PI

abx290031-400tests 400 tests
EUR 309.6

pI

EF007077 96 Tests
EUR 826.8

StemBoost? SMAD Signaling Inhibitor Cocktail Set (1000X), Sterile-Filtered

K877-1-ML
EUR 183.6

Camptothecin (2 mg/ml)

1039-1
EUR 170.4

Kanamycin solution (50mg/ml)

MN800A-1 20 ml
EUR 152.4

LV CryoOil 1.5 ml

LVCO-1 1.5 ml
EUR 48
Description: LV CryoOil 1.5 ml

PI/ Rat PI ELISA Kit

ELA-E0379r 96 Tests
EUR 1063.2

CF350 Goat Anti-Mouse IgG1 (γ1), 2mg/mL

20245-1 50uL
EUR 178.8
Description: Minimum order quantity: 1 unit of 50uL

CF488A Goat Anti-Mouse IgG1 (γ1), 2mg/mL

20246-1 50uL
EUR 178.8
Description: Minimum order quantity: 1 unit of 50uL

CF555 Goat Anti-Mouse IgG1 (γ1), 2mg/mL

20247-1 50uL
EUR 178.8
Description: Minimum order quantity: 1 unit of 50uL

CF568 Goat Anti-Mouse IgG1 (γ1), 2mg/mL

20248-1 50uL
EUR 178.8
Description: Minimum order quantity: 1 unit of 50uL

CF594 Goat Anti-Mouse IgG1 (γ1), 2mg/mL

20249-1 50uL
EUR 178.8
Description: Minimum order quantity: 1 unit of 50uL

CF633 Goat Anti-Mouse IgG1 (γ1), 2mg/mL

20250-1 50uL
EUR 178.8
Description: Minimum order quantity: 1 unit of 50uL

CF640R Goat Anti-Mouse IgG1 (γ1), 2mg/mL

20251-1 50uL
EUR 178.8
Description: Minimum order quantity: 1 unit of 50uL

CF647 Goat Anti-Mouse IgG1 (γ1), 2mg/mL

20252-1 50uL
EUR 178.8
Description: Minimum order quantity: 1 unit of 50uL

CF680 Goat Anti-Mouse IgG1 (γ1), 2mg/mL

20253-1 50uL
EUR 193.2
Description: Minimum order quantity: 1 unit of 50uL

CF770 Goat Anti-Mouse IgG1 (γ1), 2mg/mL

20254-1 50uL
EUR 193.2
Description: Minimum order quantity: 1 unit of 50uL

CF543 Goat Anti-Mouse IgG1 (γ1), 2mg/mL

20325-1 50uL
EUR 178.8
Description: Minimum order quantity: 1 unit of 50uL

NATtrol Respiratory Pathogen Panel -1 (6 x 0.25 mL)

NATRPP-1 6 x 0.25 mL
EUR 557.95
Description: Please contact Gentaur in order to receive the datasheet of the product.

GSTP2 Glutathione S-Transferase pi 2 Mouse Recombinant Protein

PROTP46425-1 Regular: 20ug
EUR 380.4
Description: GSTP2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 210 amino acids (1-210 a.a.) and having a molecular mass of 23.5kDa.; The GSTP2 is purified by proprietary chromatographic techniques.

Random Hexamers 100 µg

26-4000-03 100 ug
EUR 150
Description:

Random Primers are a mixture of oligonucleotides representing all possible sequence for that size. Random Primers can be used to prime synthesis in oligo-labeling similar to using hexamers (1,2) and cDNA synthesis. Random prime labeling yields high specific activity labeled DNA probe which can be used for all southern, northern and in situ hybridization studies. Random Primers can be also used similar to using hexamers in cDNA synthesis in combination with oligo d(T) to yield more 5' end cDNA sequence. Recently random primers have been used to detect DNA polymorphism. These polymorphisms, simply detected as DNA segments which amplify from one parent but not the other, are inherited in a Mendelian fashion and can be used to construct genetic maps in a variety of species. The authors suggested that these polymorphisms be called RAPD (pronounced RAPID) makers, after Random Amplified Polymorphic DNA (3).

References 1. Feinberg, A.P. & Vogelstein, B. (1983) Anal. Biochem. 132:6-13. 2. Feinberg, A.P. & Vogelstein, B. (1984) Anal. Biochem. 137:266-267. 3. Williams J. G., Kubelik A.R., Livak K.J., Rafalski J.A. & Tingey S.V. (1990) Nucleic Acid Res. 18(22):6531-5.

Kinesis Glyphosate 1 mL 0.1 mg/mL in Water

CHR3649 1ML
EUR 310.08

Rabbit Brain Cephalin 5 mL

41052-1 5mL
EUR 224.16

AXYPREP MAG DYECLEAN KIT- 1 ML - (SAMPLE SIZE)

MAG-DYECL-1 1/pk
EUR 90
Description: Bioscience Mag Beads; Magnetic Dye

PI-103

1728-5
EUR 502.8

PI-103

1728-50 Ask for price

PI-103

A2067-100 100 mg
EUR 1005.6
Description: PI-103 is a potent and selective inhibitor of class I PI3K, mTOR and DNA-PK with IC50 values of 2, 3, 3, 15, 30 and 23 nM for p110?, p110?, p110?, p110?, mTOR and DNA-PK, respectively [1].

PI-103

A2067-25 25 mg
EUR 393.6
Description: PI-103 is a potent and selective inhibitor of class I PI3K, mTOR and DNA-PK with IC50 values of 2, 3, 3, 15, 30 and 23 nM for p110?, p110?, p110?, p110?, mTOR and DNA-PK, respectively [1].

PI-103

A2067-5 5 mg
EUR 176.4
Description: PI-103 is a potent and selective inhibitor of class I PI3K, mTOR and DNA-PK with IC50 values of 2, 3, 3, 15, 30 and 23 nM for p110?, p110?, p110?, p110?, mTOR and DNA-PK, respectively [1].

PI-103

A2067-5.1 10 mM (in 1mL DMSO)
EUR 184.8
Description: PI-103 is a potent and selective inhibitor of class I PI3K, mTOR and DNA-PK with IC50 values of 2, 3, 3, 15, 30 and 23 nM for p110?, p110?, p110?, p110?, mTOR and DNA-PK, respectively [1].

PI-103

A2067-S Evaluation Sample
EUR 97.2
Description: PI-103 is a potent and selective inhibitor of class I PI3K, mTOR and DNA-PK with IC50 values of 2, 3, 3, 15, 30 and 23 nM for p110?, p110?, p110?, p110?, mTOR and DNA-PK, respectively [1].

PI / RNAse

abx290029-200tests 200 tests
EUR 326.4

PI-3065

B4660-10 10 mg
EUR 268.8
Description: PI-3065 is a small molecule and selective inhibitor of p110?kinase with an Ki value of 1.5nM and IC50 value of 5nM [1].PI-3065 has been reported to selectively inhibit p110?kinase with Ki values of 15nM, 110nM, 130nM and 940nM

PI-3065

B4660-100 100 mg
EUR 950.4
Description: PI-3065 is a small molecule and selective inhibitor of p110?kinase with an Ki value of 1.5nM and IC50 value of 5nM [1].PI-3065 has been reported to selectively inhibit p110?kinase with Ki values of 15nM, 110nM, 130nM and 940nM

PI-3065

B4660-5 5 mg
EUR 199.2
Description: PI-3065 is a small molecule and selective inhibitor of p110?kinase with an Ki value of 1.5nM and IC50 value of 5nM [1].PI-3065 has been reported to selectively inhibit p110?kinase with Ki values of 15nM, 110nM, 130nM and 940nM

PI-3065

B4660-5.1 10 mM (in 1mL DMSO)
EUR 212.4
Description: PI-3065 is a small molecule and selective inhibitor of p110?kinase with an Ki value of 1.5nM and IC50 value of 5nM [1].PI-3065 has been reported to selectively inhibit p110?kinase with Ki values of 15nM, 110nM, 130nM and 940nM

PI-3065

B4660-50 50 mg
EUR 686.4
Description: PI-3065 is a small molecule and selective inhibitor of p110?kinase with an Ki value of 1.5nM and IC50 value of 5nM [1].PI-3065 has been reported to selectively inhibit p110?kinase with Ki values of 15nM, 110nM, 130nM and 940nM

PI-1840

B4755-10 10 mg
EUR 220.8
Description: PI-1840 is a potent and selective inhibitor of proteasome with IC50 value of 27 nM for chymotrypsinlike (CT-L) activity [1]. The 26S proteasome consists of a 19S regulatory particle (RP) and a 20S core particle.

PI-1840

B4755-50 50 mg
EUR 626.4
Description: PI-1840 is a potent and selective inhibitor of proteasome with IC50 value of 27 nM for chymotrypsinlike (CT-L) activity [1]. The 26S proteasome consists of a 19S regulatory particle (RP) and a 20S core particle.

PI-3065

B1674-25
EUR 607.2

PI-3065

B1674-5
EUR 189.6

PI 828

B7198-10 10 mg
EUR 381.6

PI 828

B7198-5 5 mg
EUR 243.6

PI-103

E1KS1038 5mg
EUR 390

PI-103

HY-10115 100mg
EUR 655.2

PI-828

HY-108606 1mg
EUR 170.4

PI-3065

HY-12235 10mM/1mL
EUR 244.8

PI-1840

HY-12286 50mg
EUR 704.4

Arachidonic Acid 5mg/ml

ABP-ARA-1 3 x 0,5ml vials
EUR 68.4

AAVancedTM Concentration Reagent (100 ml aliquot)

AAV100A-1 100 ml
EUR 625.2

AAVancedTM Concentration Reagent (250 ml aliquot)

AAV110A-1 250 ml
EUR 1198.8

ExoQuick exosome precipitation solution (20 ml)

EXOQ20A-1 20 ml
EUR 1414.8

ExoQuick exosome precipitation solution (5 ml)

EXOQ5A-1 5 ml
EUR 480

CDC45 Antibody / PORC-PI-1

RQ4295 100 ug
EUR 419

CDC45 Antibody / PORC-PI-1

RQ4490 100ul
EUR 419

CDC45 Antibody / PORC-PI-1

RQ6278 100 ug
EUR 419
Description: CDC45 is a protein that in humans is encoded by the CDC45 gene. The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants.

Histone H3 Methylation Antibody Panel Pack I – Active Genes

C10000
  • EUR 928.26
  • EUR 588.50
  • 4 x 25 µg
  • 4 x 25 ul

Histone H3 Methylation Antibody Panel Pack I – Repression Genes

C10001
  • EUR 928.26
  • EUR 588.50
  • 4 x 25 µg
  • 4 x 25 ul

Histone H3 Methylation Antibody Panel Pack II – Active Genes

C10002
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H3 Methylation Antibody Panel Pack II – Repression Genes

C10003
  • EUR 928.26
  • EUR 588.50
  • 4 x 25 µg
  • 4 x 25 ul

Histone H3 Methylation Antibody Panel Pack III – Active Genes

C10004
  • EUR 928.26
  • EUR 588.50
  • 4 x 25 µg
  • 4 x 25 ul

Histone H3K4 Methylation Antibody Panel Pack

C10005
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Histone H3K9 Methylation Antibody Panel Pack

C10006
  • EUR 754.26
  • EUR 470.80
  • 3 x 25 µg
  • 3 x 25 ul

Kvantifikace mRNA pomocí RT-PCR v reálném čase. 

The actual-time reverse transcription polymerase chain response (RT-qPCR) addresses the evident requirement for quantitative knowledge evaluation in molecular drugs, biotechnology, microbiology and diagnostics and has develop into the strategy of alternative for the quantification of mRNA. Though it’s typically described as a “gold” commonplace, it’s removed from being a regular assay. The numerous issues brought on by variability of RNA templates, assay designs and protocols, in addition to inappropriate knowledge normalization and inconsistent knowledge evaluation, are broadly recognized but in addition broadly disregarded.

As a primary step in the direction of standardization, we describe a collection of RT-qPCR protocols that illustrate the important technical steps required to generate quantitative knowledge which can be dependable and reproducible. We want to emphasize, nonetheless, that RT-qPCR knowledge represent solely a snapshot of data concerning the amount of a given transcript in a cell or tissue. Any evaluation of the organic penalties of variable mRNA ranges should embrace extra data concerning regulatory RNAs, protein ranges and protein exercise. All the protocol described right here, encompassing all phases from preliminary assay design to dependable qPCR knowledge evaluation, requires roughly 15 h.

NCBI GEO: archiv professional funkční datové soubory genomiky – po 10 letech. 

A decade in the past, the Gene Expression Omnibus (GEO) database was established on the Nationwide Heart for Biotechnology Info (NCBI). The unique goal of GEO was to function a public repository for high-throughput gene expression knowledge generated largely by microarray expertise. Nevertheless, the analysis neighborhood shortly utilized microarrays to non-gene-expression research, together with examination of genome copy quantity variation and genome-wide profiling of DNA-binding proteins. As a result of the GEO database was designed with a versatile construction, it was potential to shortly adapt the repository to retailer these knowledge varieties. Extra lately, because the microarray neighborhood switches to next-generation sequencing applied sciences, GEO has once more tailored to host these knowledge units.

At the moment, GEO shops over 20,000 microarray- and sequence-based purposeful genomics research, and continues to deal with nearly all of direct high-throughput knowledge submissions from the analysis neighborhood. A number of mechanisms are supplied to assist customers successfully search, browse, obtain and visualize the information on the degree of particular person genes or whole research. This paper describes latest database enhancements, together with new search and knowledge illustration instruments, in addition to a short assessment of how the neighborhood makes use of GEO knowledge.

Vývoj řady binárních vektorů brány, pGWB, professional realizaci efektivní konstrukce fúzních genů professional transformaci rostlin. 

  • We developed a brand new collection of binary vectors helpful for Gateway cloning to facilitate transgenic experiments in plant biotechnology. The brand new system, Gateway Binary Vectors (pGWBs) realized environment friendly cloning, constitutive expression utilizing the cauliflower mosaic virus (CaMV) 35S promoter and the development of fusion genes by easy clonase response with an entry clone.
  • The reporters employable on this system are beta-glucuronidase (GUS), artificial inexperienced fluorescent protein with S65T mutation (sGFP), luciferase (LUC), enhanced yellow fluorescent protein (EYFP), and enhanced cyan fluorescent protein (ECFP). The tags accessible are 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7-epitope, and tandem affinity purification (TAP). In complete, 13 sorts of reporter or tag have been organized and have been nearly relevant to each N- and C-fusions.
  • The pGWBs could possibly be used for a lot of functions, reminiscent of promoter::reporter evaluation, statement of subcellular localization by the expression of proteins fused to a reporter or tag, and evaluation of protein-protein interplay by copurification and immunodetection experiments.
  • The pGWBs have been constructed with modified pBI101 containing a CaMV35S promoter-driven hygromycin phosphotransferase (HPT) gene because the second choice marker. We additionally constructed pGWBs with the marker HPT pushed by the nopaline synthase promoter. Through the use of the pGWB system, the expression of tagged proteins, and the localization of GFP-fused proteins have been simply analyzed. Furthermore, tissue-specific and inducible gene expression utilizing a promoter was additionally monitored with pGWBs. It’s anticipated that, the pGWB system will function a robust device for plasmid development in plant analysis.

NCBI GEO: archiv professional vysoce výkonná funkční genomová knowledge.

 The Gene Expression Omnibus (GEO) on the Nationwide Heart for Biotechnology Info (NCBI) is the most important public repository for high-throughput gene expression knowledge. Moreover, GEO hosts different classes of high-throughput purposeful genomic knowledge, together with people who study genome copy quantity variations, chromatin construction, methylation standing and transcription issue binding. These knowledge are generated by the analysis neighborhood utilizing high-throughput applied sciences like microarrays and, extra lately, next-generation sequencing.

The database has a versatile infrastructure that may seize absolutely annotated uncooked and processed knowledge, enabling compliance with main community-derived scientific reporting requirements reminiscent of ‘Minimal Info A few Microarray Experiment’ (MIAME). Along with serving as a centralized knowledge storage hub, GEO affords many instruments and options that permit customers to successfully discover, analyze and obtain expression knowledge from each gene-centric and experiment-centric views. This text summarizes the GEO repository construction, content material and working procedures, in addition to lately launched knowledge mining options.

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