• 30 května, 2021

Alidea s.r.o.

Vše o českých biotechnologiích

alidea
epigenetic polyclonals tissues

Abirateron u metastazujícího karcinomu prostaty

Abirateron u metastazujícího karcinomu prostaty bez předchozí chemoterapie.

Abiraterone acetate, an androgen biosynthesis inhibitor, improves total survival in sufferers with metastatic castration-resistant prostate most cancers after chemotherapy. We evaluated this agent in sufferers who had not acquired earlier chemotherapy.

METHODS

On this double-blind examine, we randomly assigned 1088 sufferers to obtain abiraterone acetate (1000 mg) plus prednisone (5 mg twice day by day) or placebo plus prednisone. The coprimary finish factors have been radiographic progression-free survival and total survival.

RESULTS

The examine was unblinded after a deliberate interim evaluation that was carried out after 43% of the anticipated deaths had occurred. The median radiographic progression-free survival was 16.5 months with abiraterone-prednisone and eight.three months with prednisone alone (hazard ratio for abiraterone-prednisone vs. prednisone alone, 0.53; 95% confidence interval [CI], 0.45 to 0.62; P<0.001). Over a median follow-up interval of 22.2 months, total survival was improved with abiraterone-prednisone (median not reached, vs. 27.2 months for prednisone alone; hazard ratio, 0.75; 95% CI, 0.61 to 0.93; P=0.01) however didn’t cross the efficacy boundary.

Abiraterone-prednisone confirmed superiority over prednisone alone with respect to time to initiation of cytotoxic chemotherapy, opiate use for cancer-related ache, prostate-specific antigen development, and decline in efficiency standing. Grade three or four mineralocorticoid-related adversarial occasions and abnormalities on liver-function testing have been extra widespread with abiraterone-prednisone.

CONCLUSIONS

Abiraterone improved radiographic progression-free survival, confirmed a pattern towards improved total survival, and considerably delayed scientific decline and initiation of chemotherapy in sufferers with metastatic castration-resistant prostate most cancers. (Funded by Janssen Analysis and Improvement, previously Cougar Biotechnology; ClinicalTrials.gov quantity, NCT00887198.).

 

alidea
alidea

Fluazifop solution 10 µg/ml in ethyl acetate
GW2901-2 2
EUR 126.2

Fluoroshield™ with PI
AR-6502-01 20 ml
EUR 68.05

Fluoroshield™ with PI
AR-6502-02 100ml
EUR 247.1

GFP | Green Fluorescence Protein - ALP conjugated 40 µg
AS20-4443-ALP 40 µg
EUR 406

GFP | Green Fluorescence Protein - HRP conjugated 40 µg
AS20-4443-HRP 40 µg
EUR 406

GFP | Green Fluorescence Protein - Biotin conjugated 40 µg
AS20-4443B 40 µg
EUR 406

Annexin V-Alexa Fluor 647/PI Apoptosis Detection Kit Annexin V-Alexa Fluor 647/PI
40304ES20 20 T
EUR 242

Annexin V-Alexa Fluor 647/PI Apoptosis Detection Kit Annexin V-Alexa Fluor 647/PI
40304ES50 50 T
EUR 442

Annexin V-Alexa Fluor 647/PI Apoptosis Detection Kit Annexin V-Alexa Fluor 647/PI
40304ES60 100 T
EUR 642

Annexin V-Alexa Fluor 488/PI Apoptosis Detection Kit Annexin V-Alexa Fluor 488/PI
40305ES20 20 T
EUR 242

Annexin V-Alexa Fluor 488/PI Apoptosis Detection Kit Annexin V-Alexa Fluor 488/PI
40305ES50 50 T
EUR 442

Annexin V-Alexa Fluor 488/PI Apoptosis Detection Kit Annexin V-Alexa Fluor 488/PI
40305ES60 100 T
EUR 642

GFP | Green fluorescent protein (VENUS) - ALP conjugated 40 µg
AS18-4227-ALP 40 µg
EUR 406

GFP | Green fluorescent protein (VENUS) - HRP conjugated 40 µg
AS18-4227-HRP 40 µg
EUR 406

GFP | Green fluorescent protein (VENUS) - Biotin conjugated 40 µg
AS18-4227B 40 µg
EUR 406

PAH Std 1-Fluoropyrene 100ug/mL in Toluene - 1ML
REPAH9012-I 1ML
EUR 411.26

PAH Std 1-Fluorochrysene 100ug/mL in Toluene - 1ML
REPAH9013-I 1ML
EUR 721.18

Colcemid Solution (10 µg/ml) in DPBS - 10 ml
CACOL-H each
EUR 13.58

1000 ug/mL Fluorenone in Methanol - 1ML
S-4332 1ML
EUR 128.25

Annexin V-Alexa Fluor488/PI Apoptosis Detection Kit
E28LS4001-100 100T
EUR 793.65

Annexin V-Alexa Fluor488/PI Apoptosis Detection Kit
E28LS4001-20 20T
EUR 279.37

Annexin V-Alexa Fluor488/PI Apoptosis Detection Kit
E28LS4001-50 50T
EUR 507.94

Annexin V-Alexa Fluor647/PI Apoptosis Detection Kit
E28LS4002-100 100T
EUR 793.65

Annexin V-Alexa Fluor647/PI Apoptosis Detection Kit
E28LS4002-20 20T
EUR 279.37

Annexin V-Alexa Fluor647/PI Apoptosis Detection Kit
E28LS4002-50 50T
EUR 507.94

Annexin V-Elab Fluor® 647/PI Apoptosis Kit
E-CK-A213-100Assays 100 Assays
EUR 260
Description: Apoptosis/Annexin V

Annexin V-Elab Fluor® 647/PI Apoptosis Kit
E-CK-A213-200Assays 200 Assays
EUR 398
Description: Apoptosis/Annexin V

Annexin V-Elab Fluor® 647/PI Apoptosis Kit
E-CK-A213-20Assays 20 Assays
EUR 75
Description: Apoptosis/Annexin V

Annexin V-Elab Fluor® 647/PI Apoptosis Kit
E-CK-A213-50Assays 50 Assays
EUR 150
Description: Apoptosis/Annexin V

Annexin V-Elab Fluor® 488/PI Apoptosis Kit
E-CK-A237-100Assays 100 Assays
EUR 260
Description: Apoptosis/Annexin V

Annexin V-Elab Fluor® 488/PI Apoptosis Kit
E-CK-A237-200Assays 200 Assays
EUR 398
Description: Apoptosis/Annexin V

Annexin V-Elab Fluor® 488/PI Apoptosis Kit
E-CK-A237-20Assays 20 Assays
EUR 75
Description: Apoptosis/Annexin V

Annexin V-Elab Fluor® 488/PI Apoptosis Kit
E-CK-A237-50Assays 50 Assays
EUR 150
Description: Apoptosis/Annexin V

Colcemid 10 µg/ml in PBS (Demecolcin) - 10ml
LM-T1700/10 10ml
EUR 2.86

Colcemid 10 µg/ml in PBS (Demecolcin) - 20ml
LM-T1700/20 20ml
EUR 6.66

Colcemid 10 µg/ml in PBS (Demecolcin) - 50ml
LM-T1700/50 50ml
EUR 10.67

4-Fluoroisobutyrylfentanyl (1.0mg/ml in Acetonitrile)
KIT4735 10x1ml
EUR 161
Description: 244195-32-2

Annexin V-Alexa Fluor 488/PI Apoptosis Detection Kit
DLSM0244 100 Assays Ask for price
Description: Detection phosphatidylserine on the outer leaflet of the cell membrane using flow cytometry.

Annexin V-Alexa Fluor 647/PI Apoptosis Detection Kit
DLSM0245 100 Assays Ask for price
Description: Detection phosphatidylserine on the outer leaflet of the cell membrane using flow cytometry.

Annexin V-Alexa Fluor 488/PI Apoptosis Detection Kit
MBS8305408-100Assays 100Assays
EUR 420

Annexin V-Alexa Fluor 488/PI Apoptosis Detection Kit
MBS8305408-50Assays 50Assays
EUR 365

Annexin V-Alexa Fluor 488/PI Apoptosis Detection Kit
MBS8305408-5x100Assays 5x100Assays
EUR 1830

Annexin V-Alexa Fluor 647/PI Apoptosis Detection Kit
MBS8305409-100Assays 100Assays
EUR 420

Annexin V-Alexa Fluor 647/PI Apoptosis Detection Kit
MBS8305409-50Assays 50Assays
EUR 365

Annexin V-Alexa Fluor 647/PI Apoptosis Detection Kit
MBS8305409-5x100Assays 5x100Assays
EUR 1830

Annexin V-Alexa Fluor 488/PI Apoptosis Detection Kit
RDSM244 100 Assays
EUR 350
Description: Detection phosphatidylserine on the outer leaflet of the cell membrane using flow cytometry.

Annexin V-Alexa Fluor 647/PI Apoptosis Detection Kit
RDSM245 100 Assays
EUR 350
Description: Detection phosphatidylserine on the outer leaflet of the cell membrane using flow cytometry.

PAH Std Fluorene 2000ug/mL in Toluene - 1ML
REPAH106 1ML
EUR 65.66

PAH Std Fluoranthene 1000ug/mL in DCM - 1ML
REPAH158 1ML
EUR 67.12

PAH Std Fluoranthene 2000ug/mL in DCM - 1ML
REPAH159 1ML
EUR 82.25

METAFECTENE® FluoR 1 x 1 ml
T050-1.0 1 ml
EUR 187.6

PAH Std Fluoroanthene 2000ug/mL in Toluene - 1ML
REPAH105 1ML
EUR 65.66

PAH Std 9-Fluorobenzo(k)fluoranthene 100ug/mL in Toluene - 1ML
REPAH9016-I 1ML
EUR 1545.18

4-Fluoroephedrone Hydrochloride (1mg/ml in Methanol)
KIT1225 5x1ml
EUR 800
Description: 7589-35-7

Sodium Fluoride, 1 M, 100 ML
M-CSS-290 100 ml
EUR 94
Description: Sodium Fluoride, 1 M, 100 ML

2-Fluorodiphenyl Ether 50ug/mL in Isooctane - 1ML
REPBDE0300 1ML
EUR 608.08

4-Fluorodiphenyl Ether 50ug/mL in Isooctane - 1ML
REPBDE0301 1ML
EUR 608.08

VOC Int Std Fluorobenzene 2000ug/mL in MeOH - 1ML
REVOC001-I 1ML
EUR 180.66

VOC Int Std Fluorobenzene 1000ug/mL in MeOH - 1ML
REVOC002-I 1ML
EUR 139.54

VOC Int Std Fluorobenzene 4000ug/mL in MeOH - 1ML
REVOC003-I 1ML
EUR 70.36

Hexachloro-1,3-butadiene-13C4 (100 µg/mL in Isooctane)
H295148 5mg
EUR 165
Description: 93951-70-3

PAH Std 2-Fluorofluorene 100ug/mL in Toluene - 1ML
REPAH9004-I 1ML
EUR 146.88

PAH Std 3-Fluorochrysene 100ug/mL in Toluene - 1ML
REPAH9014-I 1ML
EUR 358.39

Annexin V-Elab Fluor® Red 780/PI Apoptosis Kit
E-CK-A239-100Assays 100 Assays
EUR 260
Description: Apoptosis/Annexin V

Annexin V-Elab Fluor® Red 780/PI Apoptosis Kit
E-CK-A239-200Assays 200 Assays
EUR 398
Description: Apoptosis/Annexin V

Annexin V-Elab Fluor® Red 780/PI Apoptosis Kit
E-CK-A239-20Assays 20 Assays
EUR 75
Description: Apoptosis/Annexin V

Annexin V-Elab Fluor® Red 780/PI Apoptosis Kit
E-CK-A239-50Assays 50 Assays
EUR 150
Description: Apoptosis/Annexin V

VOC Int Std 2-Bromo-1-Chloropropane Fluorobenzene 1000ug/mL in MeOH - 1ML
REVOC005-I 1ML
EUR 124.85

PAH Std 2-Fluorophenanthrene 100ug/mL in Toluene - 1ML
REPAH9007-I 1ML
EUR 358.39

PAH Std 3-Fluorophenanthrene 100ug/mL in Toluene - 1ML
REPAH9008-I 1ML
EUR 358.39

PAH Std 4-Fluorophenanthrene 100ug/mL in Toluene - 1ML
REPAH9009-I 1ML
EUR 556.68

PAH Std 3-Fluorofluoranthene 100ug/mL in Toluene - 1ML
REPAH9011-I 1ML
EUR 146.88

METAFECTENE® FluoR 1 x 0.5 ml
T050-0.5 0.5 ml
EUR 108.5

PAH Std Benzo(b)fluoranthene 2000ug/ml in MeOH - 1ML
REPAH145 1ML
EUR 180.66

PAH Std 5-Fluoroacenaphthylene 100ug/mL in Toluene - 1ML
REPAH9002-I 1ML
EUR 500.86

Annexin V-Elab Fluor® Violet 450/PI Apoptosis Kit
E-CK-A233-100Assays 100 Assays
EUR 260
Description: Apoptosis/Annexin V

Annexin V-Elab Fluor® Violet 450/PI Apoptosis Kit
E-CK-A233-200Assays 200 Assays
EUR 398
Description: Apoptosis/Annexin V

Annexin V-Elab Fluor® Violet 450/PI Apoptosis Kit
E-CK-A233-20Assays 20 Assays
EUR 75
Description: Apoptosis/Annexin V

Annexin V-Elab Fluor® Violet 450/PI Apoptosis Kit
E-CK-A233-50Assays 50 Assays
EUR 150
Description: Apoptosis/Annexin V

Annexin V-Elab Fluor® Violet 500/PI Apoptosis Kit
E-CK-A235-100Assays 100 Assays
EUR 260
Description: Apoptosis/Annexin V

Annexin V-Elab Fluor® Violet 500/PI Apoptosis Kit
E-CK-A235-200Assays 200 Assays
EUR 398
Description: Apoptosis/Annexin V

Annexin V-Elab Fluor® Violet 500/PI Apoptosis Kit
E-CK-A235-20Assays 20 Assays
EUR 75
Description: Apoptosis/Annexin V

Annexin V-Elab Fluor® Violet 500/PI Apoptosis Kit
E-CK-A235-50Assays 50 Assays
EUR 150
Description: Apoptosis/Annexin V

PAH Std 4-Fluorodiphenylmethane 100ug/mL in Toluene - 1ML
REPAH9003-I 1ML
EUR 189.48

PAH Std 2-Fluorodiphenylmethane 100ug/mL in Toluene - 1ML
REPAH9005-I 1ML
EUR 189.48

PAH Std 2-Fluorodibenzothiophene 100ug/mL in Toluene - 1ML
REPAH7003 1ML
EUR 1106.01

PAH Std Benzo(k)fluoranthene 2000ug/ml in Toluene - 1ML
REPAH1141 1ML
EUR 165.97

PAH Std Benzo(a)fluoranthene 200ug/mL in Toluene - 1ML
REPAH2401 1ML
EUR 1286.67

PAH Std Benzo(k)fluoroanthene 2000ug/mL in Toluene - 1ML
REPAH114 1ML
EUR 67.12

PAH Std Benzo(ghi)fluoranthene 200ug/mL in Toluene - 1ML
REPAH2402 1ML
EUR 1600.99

Kvantifikace mRNA pomocí RT-PCR v reálném čase. 

The actual-time reverse transcription polymerase chain response (RT-qPCR) addresses the evident requirement for quantitative knowledge evaluation in molecular drugs, biotechnology, microbiology and diagnostics and has develop into the strategy of alternative for the quantification of mRNA. Though it’s typically described as a “gold” commonplace, it’s removed from being a regular assay. The numerous issues brought on by variability of RNA templates, assay designs and protocols, in addition to inappropriate knowledge normalization and inconsistent knowledge evaluation, are broadly recognized but in addition broadly disregarded.

As a primary step in the direction of standardization, we describe a collection of RT-qPCR protocols that illustrate the important technical steps required to generate quantitative knowledge which can be dependable and reproducible. We want to emphasize, nonetheless, that RT-qPCR knowledge represent solely a snapshot of data concerning the amount of a given transcript in a cell or tissue. Any evaluation of the organic penalties of variable mRNA ranges should embrace extra data concerning regulatory RNAs, protein ranges and protein exercise. All the protocol described right here, encompassing all phases from preliminary assay design to dependable qPCR knowledge evaluation, requires roughly 15 h.

NCBI GEO: archiv professional funkční datové soubory genomiky – po 10 letech. 

A decade in the past, the Gene Expression Omnibus (GEO) database was established on the Nationwide Heart for Biotechnology Info (NCBI). The unique goal of GEO was to function a public repository for high-throughput gene expression knowledge generated largely by microarray expertise. Nevertheless, the analysis neighborhood shortly utilized microarrays to non-gene-expression research, together with examination of genome copy quantity variation and genome-wide profiling of DNA-binding proteins. As a result of the GEO database was designed with a versatile construction, it was potential to shortly adapt the repository to retailer these knowledge varieties. Extra lately, because the microarray neighborhood switches to next-generation sequencing applied sciences, GEO has once more tailored to host these knowledge units.

At the moment, GEO shops over 20,000 microarray- and sequence-based purposeful genomics research, and continues to deal with nearly all of direct high-throughput knowledge submissions from the analysis neighborhood. A number of mechanisms are supplied to assist customers successfully search, browse, obtain and visualize the information on the degree of particular person genes or whole research. This paper describes latest database enhancements, together with new search and knowledge illustration instruments, in addition to a short assessment of how the neighborhood makes use of GEO knowledge.

Vývoj řady binárních vektorů brány, pGWB, professional realizaci efektivní konstrukce fúzních genů professional transformaci rostlin. 

  • We developed a brand new collection of binary vectors helpful for Gateway cloning to facilitate transgenic experiments in plant biotechnology. The brand new system, Gateway Binary Vectors (pGWBs) realized environment friendly cloning, constitutive expression utilizing the cauliflower mosaic virus (CaMV) 35S promoter and the development of fusion genes by easy clonase response with an entry clone.
  • The reporters employable on this system are beta-glucuronidase (GUS), artificial inexperienced fluorescent protein with S65T mutation (sGFP), luciferase (LUC), enhanced yellow fluorescent protein (EYFP), and enhanced cyan fluorescent protein (ECFP). The tags accessible are 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7-epitope, and tandem affinity purification (TAP). In complete, 13 sorts of reporter or tag have been organized and have been nearly relevant to each N- and C-fusions.
  • The pGWBs could possibly be used for a lot of functions, reminiscent of promoter::reporter evaluation, statement of subcellular localization by the expression of proteins fused to a reporter or tag, and evaluation of protein-protein interplay by copurification and immunodetection experiments.
  • The pGWBs have been constructed with modified pBI101 containing a CaMV35S promoter-driven hygromycin phosphotransferase (HPT) gene because the second choice marker. We additionally constructed pGWBs with the marker HPT pushed by the nopaline synthase promoter. Through the use of the pGWB system, the expression of tagged proteins, and the localization of GFP-fused proteins have been simply analyzed. Furthermore, tissue-specific and inducible gene expression utilizing a promoter was additionally monitored with pGWBs. It’s anticipated that, the pGWB system will function a robust device for plasmid development in plant analysis.

NCBI GEO: archiv professional vysoce výkonná funkční genomová knowledge.

 The Gene Expression Omnibus (GEO) on the Nationwide Heart for Biotechnology Info (NCBI) is the most important public repository for high-throughput gene expression knowledge. Moreover, GEO hosts different classes of high-throughput purposeful genomic knowledge, together with people who study genome copy quantity variations, chromatin construction, methylation standing and transcription issue binding. These knowledge are generated by the analysis neighborhood utilizing high-throughput applied sciences like microarrays and, extra lately, next-generation sequencing.

The database has a versatile infrastructure that may seize absolutely annotated uncooked and processed knowledge, enabling compliance with main community-derived scientific reporting requirements reminiscent of ‘Minimal Info A few Microarray Experiment’ (MIAME). Along with serving as a centralized knowledge storage hub, GEO affords many instruments and options that permit customers to successfully discover, analyze and obtain expression knowledge from each gene-centric and experiment-centric views. This text summarizes the GEO repository construction, content material and working procedures, in addition to lately launched knowledge mining options.

Štítky:
alidea
alidea

Napsat komentář

Vaše e-mailová adresa nebude zveřejněna. Vyžadované informace jsou označeny *